Document Detail


Oxidized low-density lipoprotein (Ox-LDL) impacts on erythrocyte viscoelasticity and its molecular mechanism.
MedLine Citation:
PMID:  19747680     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oxidized low-density lipoprotein (Ox-LDL) plays an important role in atherosclerosis, yet it remains unclear if it damages circulating erythrocytes. In this study, erythrocyte deformability and its membrane proteins after Ox-LDL incubations are investigated by micropipette aspiration, thiol radical measurement, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Results show that Ox-LDL incubation reduces the erythrocyte deformability, decreases free thiol radical contents in erythrocytes, and induces the cross-linking among membrane proteins. SDS-PAGE analysis reveals a high molecular weight (HMW) complex as well as new bands between spectrins and band 3 and reduced ratios between band 3 and other major membrane skeletal proteins. Analyses indicate that Ox-LDL makes erythrocytes harder to deform through a molecular mechanism by which the oxidation of free thiol radicals forms disulfide bonds among membrane skeletal proteins.
Authors:
Xiang Wang; Li Yang; Yao Liu; Wei Gao; Weiyan Peng; K-L Paul Sung; Lanping Amy Sung
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-10
Journal Detail:
Title:  Journal of biomechanics     Volume:  42     ISSN:  1873-2380     ISO Abbreviation:  J Biomech     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2010-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0157375     Medline TA:  J Biomech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2394-9     Citation Subset:  IM    
Affiliation:
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing 400044, PR China. xwangchn@cqu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Computer Simulation
Elastic Modulus / drug effects,  physiology
Erythrocytes / drug effects*,  physiology*
Humans
Lipoproteins, LDL / pharmacology*
Models, Cardiovascular*
Viscosity / drug effects
Chemical
Reg. No./Substance:
0/Lipoproteins, LDL; 0/oxidized low density lipoprotein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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