| Oxidized chondroitin sulfate-cross-linked gelatin matrixes: a new class of hydrogels. | |
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MedLine Citation:
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PMID: 16004443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A naturally occurring glycosaminoglycan such as chondroitin-6-sulfate was first converted in to its aldehyde derivative by periodate oxidation and used as a cross-linking agent for gelatin giving rise to a new class of hydrogels. Cross-linking was predominantly due to Schiff's base formation between the epsilon-amino groups of lysine or hydroxylysine side groups of gelatin and the aldehyde groups in oxidized chondroitin sulfate. The hydrogels were prepared from chondroitin sulfate with different degrees of oxidation and gelatin. They were characterized for degree of cross-linking, cross-linking density, equilibrium swelling, water vapor transmission rate, internal structure, and blood-compatibility. Degree of cross-linking of the gels determined by trinitrobenzene sulfonic acid assay showed that, the higher the degree of oxidation of the polysaccharide, the higher the degree of cross-linking. Examination of the internal structure by scanning electron microscopy showed that the hydrogels were highly porous in nature with interconnecting pores ranging from 50 to 200 mum. Equilibrium swelling showed that the gels retained about 90% water and did not undergo dehydration rapidly. The hydrogels were nontoxic and blood-compatible. Since an important phase of early wound healing has been shown to involve secretion of glycosaminoglycans such as chondroitin sulfate by fibroblasts which form a hydrophilic matrix suitable for remodeling during healing, this new class of hydrogels prepared from chondroitin sulfate and gelatin without employing any extraneous cross-linking agents are expected to have potential as wound dressing materials. |
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Authors:
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S Dawlee; A Sugandhi; Biji Balakrishnan; D Labarre; A Jayakrishnan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomacromolecules Volume: 6 ISSN: 1525-7797 ISO Abbreviation: Biomacromolecules Publication Date: 2005 Jul-Aug |
Date Detail:
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Created Date: 2005-07-11 Completed Date: 2005-11-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 2040-8 Citation Subset: IM |
Affiliation:
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Polymer Chemistry Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Satelmond Palace, Poojapura, Trivandrum, Kerala 695 012, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Chondroitin Sulfates / chemistry* Cross-Linking Reagents / chemistry* Gelatin / chemistry* Hydrogels / chemistry* Microscopy, Electron, Scanning Oxidation-Reduction Periodic Acid / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Cross-Linking Reagents; 0/Hydrogels; 10450-60-9/Periodic Acid; 9000-70-8/Gelatin; 9007-28-7/Chondroitin Sulfates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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