Document Detail


Oxidative stress response in telomerase-immortalized fibroblasts from a centenarian.
MedLine Citation:
PMID:  17341606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been reported that cells with ectopic expression of telomerase are more resistant to apoptotic cell death than their normal counterpart. However, controversial results were obtained when the cellular response to oxidative stress was analyzed. The present research was therefore aimed at defining the effect of the oxidative stress induced by tert-butylhydroperoxide (tBOOH) and 2-deoxy-D-ribose (D-ribose) in human fibroblasts from a centenarian (cen3) and, in parallel, on the same cells after telomerase immortalization (cen3tel cells). By studying different parameters of apoptosis in situ (i.e., chromatin condensation, phosphatidylserine externalization, and DNA fragmentation), we found that both tBOOH and D-ribose induce apoptosis to a greater extent in cen3 than in cen3tel cells, suggesting a protective role of telomerase toward apoptotic death. However, monitoring the cell number during treatment with the drugs, we found a decrease in cell number; since this reduction was lower in cen3 fibroblasts compared to cen3tel cells, it is likely that telomerase does not fully protect cells from drug toxicity.
Authors:
Chiara Mondello; Maria Grazia Bottone; Sakon Noriki; Cristiana Soldani; Carlo Pellicciari; Anna Ivana Scovassi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1091     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2007-03-07     Completed Date:  2007-03-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-101     Citation Subset:  IM    
Affiliation:
Istituto di Genetica Molecolare del CNR, Via Abbiategrasso 207, 27100 Pavia, Italy. mondello@igm.cnr.it
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MeSH Terms
Descriptor/Qualifier:
Aged, 80 and over
Apoptosis / drug effects,  physiology
Cell Line, Transformed
Deoxyribose / pharmacology
Fibroblasts / drug effects,  enzymology,  physiology*
Humans
Oxidative Stress / drug effects,  physiology*
Telomerase / physiology*
tert-Butylhydroperoxide / pharmacology
Chemical
Reg. No./Substance:
533-67-5/Deoxyribose; 75-91-2/tert-Butylhydroperoxide; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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