Document Detail

Oxidative stress-related parameters in the liver of non-alcoholic fatty liver disease patients.
MedLine Citation:
PMID:  14556645     Owner:  NLM     Status:  MEDLINE    
Oxidative stress is implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). In the present study, hepatic and plasma oxidative stress-related parameters were measured and correlated with clinical and histological findings in 31 NAFLD patients showing increased body mass index. Liver protein carbonyl content was enhanced by 403% in patients with steatosis (n=15) compared with control values (n=12), whereas glutathione content, superoxide dismutase (SOD) activity and the ferric reducing ability of plasma (FRAP) were decreased by 57%, 48% and 21% (P<0.05) respectively. No changes in microsomal p-nitrophenol hydroxylation and the total content of cytochrome P450 (CYP) or CYP2E1 were observed. Patients with steatohepatitis (n=16) exhibited protein carbonyl content comparable with that of controls, whereas glutathione content, SOD and catalase activities were decreased by 27%, 64% and 48% (P<0.05). In addition, FRAP values in patients with steatohepatitis were reduced by 33% and 15% (P<0.05) when compared with controls and patients with steatosis respectively, whereas p-nitrophenol hydroxylation (52%) and CYP2E1 content (142%) were significantly increased (P<0.05) compared with controls. It is concluded that oxidative stress is developed in the liver of NAFLD patients with steatosis and is exacerbated further in patients with steatohepatitis, which is associated with CYP2E1 induction. Substantial protein oxidation is followed by proteolysis of the modified proteins, which may explain the co-existence of a diminished antioxidant capacity and protein oxidation in the liver of patients with steatohepatitis.
Luis A Videla; Ramón Rodrigo; Myriam Orellana; Virginia Fernandez; Gladys Tapia; Luis Quiñones; Nelson Varela; Jorge Contreras; Raúl Lazarte; Attila Csendes; Jorge Rojas; Fernando Maluenda; Patricio Burdiles; Juan C Diaz; Gladys Smok; Lilian Thielemann; Jaime Poniachik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  106     ISSN:  0143-5221     ISO Abbreviation:  Clin. Sci.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-18     Completed Date:  2004-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  261-8     Citation Subset:  IM    
Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Casilla 70058, Santiago-7, Chile.
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MeSH Terms
Analysis of Variance
Biological Markers / analysis
Case-Control Studies
Catalase / analysis,  metabolism
Cytochrome P-450 CYP2E1 / metabolism
Fatty Liver / metabolism*,  pathology
Glutathione / analysis,  metabolism
Liver / metabolism*,  pathology
Middle Aged
Oxidative Stress*
Superoxide Dismutase / analysis,  metabolism
Reg. No./Substance:
0/Biological Markers; 70-18-8/Glutathione; EC; EC P-450 CYP2E1; EC Dismutase
Comment In:
Clin Sci (Lond). 2004 Mar;106(3):235-7   [PMID:  14604432 ]

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