Document Detail

Oxidative stress related PMK-1 P38 MAPK activation as a mechanism of toxicity of silver nanoparticles on the reproduction of the nematode Caenohabditis elegans.
MedLine Citation:
PMID:  22128035     Owner:  NLM     Status:  Publisher    
In the present study, a toxic mechanism of silver nanoparticles (AgNPs) was investigated in the nematode, Caenorhabditis elegans, focusing on the involvement of oxidative stress in reproduction toxicity. Initially, AgNPs were tested as potential oxidative stress inducers, and increased formation of reactive oxygen species (ROS) was observed in AgNPs exposed C.elegans. Subsequently, the potential upstream signaling pathway activated in response to AgNPs exposure was investigated, paying special attention to the C. elegans PMK-1 p38 mitogen-activated protein kinase (MAPK). Increased PMK-1 p38 MAPK gene and protein expressions were observed in C.elegans exposed to AgNPs. The expressions of the p38 dependent transcription factors (TFs) genes, as well as, glutathione S- transferases (GSTs) enzyme activity were also investigated in wildtype (N2) and pmk-1 mutant (km25) C. elegans exposed to AgNPs. The results indicated that AgNPs exposure lead to increased ROS formation, increased expression of PMK-1 p38 MAPK and hypoxia-inducible factor (HIF-1), GST enzyme activity, and decreased reproduction potential in wildtype (N2) C. elegans; whereas in AgNPs exposed pmk-1 (km25) mutant, ROS formation and HIF-1 and GST activation were not observed and decreased reproduction potential was rescued. These results suggest that oxidative stress is an important mechanism of AgNPs induced reproduction toxicity in C. elegans and PMK-1 p38 MAPK plays an important role in it. The results also suggest that GST and HIF-1 activation by AgNPs exposure are PMK-1 dependent, which both seem to play an important role in the PMK-1 p38 MAPK mediated defense pathway to AgNPs exposure in C. elegans. Environ. Toxicol. Chem. © 2011 SETAC.
Dongyoung Lim; Ji-Yeon Roh; Hyun-Jeong Eom; Jeongyun Choi; Jinwon Hyun; Jinhee Choi
Related Documents :
8426095 - Fibronectin cell-binding domain triggered transmembrane signal transduction in human mo...
18502815 - Cytosolic phospholipase a2alpha activation induced by s1p is mediated by the s1p3 recep...
3956485 - Differential effects of defined chemical modifications on antigenic and pharmacological...
8913345 - Involvement of mitogen-activated protein kinase and translocation of cytosolic phosphol...
9768595 - Phosphorylation of cytosolic phospholipase a2 by il-3 is associated with increased free...
2784385 - Casein kinase ii from rana temporaria oocytes. intracellular localization and activity ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-29
Journal Detail:
Title:  Environmental toxicology and chemistry / SETAC     Volume:  -     ISSN:  1552-8618     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8308958     Medline TA:  Environ Toxicol Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 SETAC.
School of Environmental Engineering, Graduate School of Energy and Environment Systems Engineering, University of Seoul, Dongdaemun-gu, Seoul, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Crohn's disease alters MHC-rafts in CD4(+) T-cells.
Next Document:  In utero exposure to maternal tobacco smoke and subsequent obesity, hypertension, and gestational di...