Document Detail

Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings.
MedLine Citation:
PMID:  11284661     Owner:  NLM     Status:  MEDLINE    
Oxidative stress is involved in the aetiology of defective embryo development. Reactive oxygen species (ROS) may originate from embryo metabolism and/or embryo surroundings. Embryo metabolism generates ROS via several enzymatic mechanisms. The relative contribution of each source seems different depending on the species, the stage of development, and the culture conditions. Several exogenous factors and culture conditions can enhance the production of ROS by embryos. ROS can alter most types of cellular molecules, and also induce development block and retardation. Multiple mechanisms of embryo protection against ROS exist, and these have complementary actions. External protection, present in follicular and tubal fluids, mainly comprises non-enzymatic antioxidants such as hypotaurine, taurine and ascorbic acid. Internal protection mainly comprises antioxidant enzymes: superoxide dismutase, glutathione peroxidase and gamma-glutamylcysteine synthetase. Transcripts encoding for these enzymes are present in the oocyte, embryo and oviduct. It may be important that these transcripts are stored during oocyte maturation in order to allow the embryo to acquire the aptitude to develop. It is now common to add antioxidant compounds to culture media. Nevertheless, maintaining the pro-oxidant-antioxidant equilibrium in embryos through such supplementation is a complex problem. Further studies are necessary to limit oxidative stress during embryo culture.
P Guérin; S El Mouatassim; Y Ménézo
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Human reproduction update     Volume:  7     ISSN:  1355-4786     ISO Abbreviation:  Hum. Reprod. Update     Publication Date:    2001 Mar-Apr
Date Detail:
Created Date:  2001-04-03     Completed Date:  2001-07-19     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  9507614     Medline TA:  Hum Reprod Update     Country:  England    
Other Details:
Languages:  eng     Pagination:  175-89     Citation Subset:  IM    
Ecole Nationale Vétérinaire de Lyon, Unité Biologie de la Reproduction, Marcy l'étoile, France.
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MeSH Terms
Antioxidants / metabolism
Blastocyst / enzymology,  physiology*
Embryonic and Fetal Development / physiology
Fertilization in Vitro
Oxidative Stress / physiology*
Reactive Oxygen Species / metabolism,  physiology*
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species

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