| Oxidative stress and liver toxicity in rats and human hepatoma cell line induced by pentachlorophenol and its major metabolite tetrachlorohydroquinone. | |
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MedLine Citation:
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PMID: 11439222 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pentachlorophenol (PCP) is a pesticide used worldwide in industrial and domestic applications. It is used extensively as biocide and wood preservatives. Metabolic studies carried out in rodents and human liver homogenates have indicated that PCP undergoes oxidative dechlorination to form tetrachlorohydroquinone (TCHQ). Free radical catalyzed tissue injury is thought to play a fundamental role in human disease. In the present study, we examined the effects of PCP and TCHQ on the induction of lipid peroxidation and liver injury in rats. In addition, the cytotoxic dose, cell death mechanisms and related gene expressions induced by PCP and TCHQ were also determined for human hepatoma cell line (Hep G2). The results indicated that more toxic effects could be observed both in rats and human hepatoma cell line treated with TCHQ than its parent compound, PCP. Oxygen species may be involved in the mechanism of TCHQ intoxication since the urinary 8-epi-PGF2alpha and AST, ALT activities can be induced by TCHQ and attenuated by vitamin E treatment. Apoptosis features were found in cells treated with TCHQ but not PCP. TCHQ-induced cell damage may issue signals for the induction of HSPs, the decrease of the bcl/bax protein ratio and the decrease of CAS gene, whereas the PCP-induced damage may not. |
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Authors:
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Y J Wang; C C Lee; W C Chang; H B Liou; Y S Ho |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Toxicology letters Volume: 122 ISSN: 0378-4274 ISO Abbreviation: Toxicol. Lett. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-07-05 Completed Date: 2001-08-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 157-69 Citation Subset: IM |
Affiliation:
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Department of Environmental and Occupational Health, National Cheng Kung University, Medical College, Tainan, Taiwan, Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine Transaminase
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blood Animals Apoptosis / drug effects Aspartate Aminotransferases / blood Cellular Apoptosis Susceptibility Protein Dinoprost / analogs & derivatives, urine F2-Isoprostanes Humans Hydroquinones / toxicity* Liver / drug effects* Male Oxidative Stress* Pentachlorophenol / toxicity* Proteins / genetics Rats Rats, Sprague-Dawley Tumor Cells, Cultured Vitamin E / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cellular Apoptosis Susceptibility Protein; 0/F2-Isoprostanes; 0/Hydroquinones; 0/Proteins; 1406-18-4/Vitamin E; 27415-26-5/8-epi-prostaglandin F2alpha; 551-11-1/Dinoprost; 87-86-5/Pentachlorophenol; 87-87-6/2,3,5,6-tetrachlorohydroquinone; EC 2.6.1.1/Aspartate Aminotransferases; EC 2.6.1.2/Alanine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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