| Oxidative stress and intracellular pH in the young and old erythrocytes of rat. | |
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MedLine Citation:
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PMID: 19214770 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of oxidative stress (OS) on the rat erythrocytes (RBCs) that were fractionated on the percoll/BSA gradient into young and old cells were studied to find out if the altered Na+/H+ and Cl⁻/ HCO3⁻ antiporters and in turn the intracellular pH (pHi) could act as one of the promoters of cell death. Old cells were more spherical with lesser surface area, more fragile osmotically and had lesser protein sulphydryl content than the young cells. OS was induced in RBCs by 2,20-azobis (2-amidinopropane) dihydrochloride (AAPH). AAPH increased the superoxide dismutase (SOD) activity and MDA level and, the changes between the young and old. Interestingly, vitamin C was effective in reducing MDA in the old. Further, in the old a rapid Na+-dependent acidification in the presence of AAPH and a marginal acidosis in the presence of vitamin C were evident. Old RBCs exhibited higher acidosis and vitamin C was less effective in lowering the stress-induced acidosis compared to the young. Our studies suggest that increased acidosis followed by low intracellular pH could be one of the determinant factors for the disappearance of old RBCs from circulation, and perhaps of the young too under OS. |
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Authors:
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S Asha Devi; C S Shiva Shankar Reddy; M V V Subramanyam |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biogerontology Volume: 10 ISSN: 1573-6768 ISO Abbreviation: Biogerontology Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2011-04-06 Completed Date: 2011-06-20 Revised Date: 2013-04-24 |
Medline Journal Info:
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Nlm Unique ID: 100930043 Medline TA: Biogerontology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 659-69 Citation Subset: IM |
Affiliation:
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Laboratory of Gerontology, Department of Zoology, Bangalore University, Bangalore 560056, Karnataka, India. asuba@blr.vsnl.net.in |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidines
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pharmacology Analysis of Variance Animals Antioxidants / pharmacology Ascorbic Acid / pharmacology Cell Aging* Chloride-Bicarbonate Antiporters / blood Erythrocytes / drug effects, metabolism* Hydrogen-Ion Concentration Lipid Peroxidation Male Malondialdehyde / blood Osmotic Fragility Oxidants / pharmacology Oxidative Stress* / drug effects Rats Sodium-Hydrogen Antiporter / blood Superoxide Dismutase / blood |
| Chemical | |
Reg. No./Substance:
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0/Amidines; 0/Antioxidants; 0/Chloride-Bicarbonate Antiporters; 0/Oxidants; 0/Sodium-Hydrogen Antiporter; 13217-66-8/2,2'-azobis(2-amidinopropane); 50-81-7/Ascorbic Acid; 542-78-9/Malondialdehyde; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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