Document Detail


Oxidative stress induces S-thiolation of specific proteins in cultured gastric mucosal cells.
MedLine Citation:
PMID:  8141298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxidative stress induces the formation of protein-mixed disulfides with low-molecular-weight thiols, especially glutathione. We analyzed this process, termed S-thiolation, in cultured gastric mucosal cells from guinea pigs by gel electrophoresis and autoradiography after radiolabeling of the intracellular glutathione pool with 35S. Hydrogen peroxide (H2O2) or diamide initiated rapid and reversible S-thiolation of specific proteins with molecular masses of 42, 30, 29, 28, and 22 kDa. Diamide caused particularly prominent S-thiolation of the 42 kDa protein. This protein was identified as actin by immunoblot analysis and actin-myosin precipitation. Fluorescence microscopy revealed that diamide caused a disappearance of normal stress fibers and a concomitant increase in actin polymerization in association with contraction of the cells. These morphological changes were completely reversible within minutes. With cells depleted of glutathione by incubation with DL-buthionine-[S,R]-sulfoximine, diamide caused severe contraction and rounding, and the cells detached from the culture plates. S-thiolation of actin could help protect gastric mucosal cells against irreversible organization of microfilaments by preserving microfilament dynamics under oxidative stress.
Authors:
K Rokutan; R B Johnston; K Kawai
Related Documents :
18846258 - The polyacetylenes falcarinol and falcarindiol affect stress responses in myotube cultu...
23941288 - In vitro batch cultures of gut microbiota from healthy and ulcerative colitis (uc) subj...
2902158 - Competitive dose-modification between ascorbate and misonidazole in human and hamster c...
18346048 - Theaflavin-3-gallate and theaflavin-3'-gallate, polyphenols in black tea with prooxidan...
18553478 - Control strategies of continuous bioprocesses based on biological activities.
7227478 - Characterization of a thrombocytopoietic-stimulating factor from kidney cell culture me...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  266     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-04-28     Completed Date:  1994-04-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G247-54     Citation Subset:  IM    
Affiliation:
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cells, Cultured
Cystine / pharmacokinetics
Diamide / pharmacology*
Gastric Mucosa / cytology,  metabolism*,  ultrastructure
Glutathione / metabolism
Guinea Pigs
Hydrogen Peroxide / pharmacology*
Intracellular Membranes / metabolism
Male
Microfilaments / ultrastructure
Microscopy, Fluorescence
Proteins / metabolism*
Sulfhydryl Compounds / metabolism*
Grant Support
ID/Acronym/Agency:
AI-24748/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Proteins; 0/Sulfhydryl Compounds; 10465-78-8/Diamide; 56-89-3/Cystine; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Role of leukocytes in indomethacin-induced small bowel injury in the rat.
Next Document:  Intrinsic corporoantropyloric coordination of motility and gastric emptying.