| Oxidative stress in mouse liver caused by dietary amino acid deprivation: protective effect of methionine. | |
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MedLine Citation:
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PMID: 20577846 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The aim of this work was to evaluate the effects of a diet depleted of amino acids (protein-free diet, or PFD), as well as the supplementation with methionine (PFD+Met), on the antioxidant status of the female mouse liver. With this purpose, cytosolic protein spots from two-dimensional non-equilibrium pH gel electrophoresis were identified by several procedures, such as mass spectrometry, Western blot, gel matching and enzymatic activity. PFD decreased the contents of catalase (CAT), peroxiredoxin I (Prx-I), and glutathione peroxidase (GPx) by 67%, 37% and 45%, respectively. Gene expression analyses showed that PFD caused a decrease in CAT (-20%) and GPx (-30%) mRNA levels but did not change that of Prx-I. It was also found that, when compared to a normal diet, PFD increased the liver contents of both reactive oxygen species (+50%) and oxidized protein (+88%) and decreased that of glutathione (-45%). Supplementation of PFD with Met prevented these latter effects to varying degrees, whereas CAT, Prx-I and GPx mRNA levels resulted unmodified. Present results suggest that dietary amino acid deprivation deranges the liver antioxidant defences, and this can be, in part, overcome by supplementation with Met. |
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Authors:
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Virginia P Ronchi; Ana M Giudici; Julieta R Mendieta; Veronica J Caballero; Andrea N Chisari; Pedro M Sanllorenti; Rubén D Conde |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-25 |
Journal Detail:
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Title: Journal of physiology and biochemistry Volume: 66 ISSN: 1138-7548 ISO Abbreviation: J. Physiol. Biochem. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9812509 Medline TA: J Physiol Biochem Country: Spain |
Other Details:
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Languages: eng Pagination: 93-103 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, CC 1245, 7600, Mar del Plata, Argentina. |
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