| Oxidative stress in cell culture: an under-appreciated problem? | |
| | |
MedLine Citation:
|
PMID: 12681474 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell culture studies have given much valuable information about mechanisms of metabolism and signal transduction and of regulation of gene expression, proliferation, senescence, and death. However, cells in culture may behave differently from cells in vivo in many ways. One of these is that cell culture imposes a state of oxidative stress on cells. I argue that cells that survive and grow in culture might use ROS-dependent signal transduction pathways that rarely or never operate in vivo. A further problem is that cell culture media can catalyse the oxidation of compounds added to them, resulting in apparent cellular effects that are in fact due to oxidation products such as ROS. Such artefacts may have affected many studies on the effects of ascorbate, thiols, flavonoids and other polyphenolic compounds on cells in culture. |
| | |
Authors:
|
Barry Halliwell |
Related Documents
:
|
9426364 - Monoisopropylglutathione ester protects a549 cells from the cytotoxic effects of sulphu... 23063554 - Element distribution in corrosion layer and cytotoxicity of mg-10dy alloy during in vit... 17363214 - Induction of reactive oxygen species and apoptosis in beas-2b cells by mercuric chloride. 20732104 - Action of bis(betachloroethyl)sulphide (bces) on human epidermis reconstituted in cultu... 16727504 - Effects of cumulus cells on male pronuclear formation and subsequent early development ... 3018294 - Coxsackievirus b3 infection alters plasma membrane of neonatal skin fibroblasts. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: FEBS letters Volume: 540 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2003 Apr |
Date Detail:
|
Created Date: 2003-04-08 Completed Date: 2003-04-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 3-6 Citation Subset: IM |
Copyright Information:
|
Copyright 2003 Federation of European Biochemical Societies |
Affiliation:
|
Department of Biochemistry, Faculty of Medicine, MD 7 #03-07, 8 Medical Drive, Singapore 117597, Singapore. bchbh@nus.edu.sg |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cells, Cultured Culture Media Oxidative Stress* Reactive Oxygen Species / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Reactive Oxygen Species |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A review of the case for hepatitis B vaccination of high-risk adults.
Next Document: The unfolding tale of PECAM-1.