Document Detail

Oxidative stress in Staphylococcus aureus associated to the cleavage of an isoxazolylnaphthoquinoneimine with antibacterial capacity.
MedLine Citation:
PMID:  9514859     Owner:  NLM     Status:  MEDLINE    
Staphylococcus aureus was inhibited by exposure to 2-hydroxy-N-(3,4-dimethyl-5-isoxazolyl)-1,4-naphthoquinone-4-imine (Q1). This compound was cleavaged in the presence of bacteria and an efflux of isoxazolamine was detected whereas in the S. aureus membrane and cytoplasm was observed an absorption band similar to that of the bencenoid ring. Non-viable bacteria showed intact Q1 intracellularly and in the membrane. Antistaphylococcus effect was associated to Q1 interaction with the respiratory chain, the oxidative metabolites were stimulated; there was cellular injury simultaneous to reduction of antibiotic molecule and efflux of isoxazolamine. The bacteria treated with Q1 increased its oxygen consumption and superoxide anion generation. Superoxide dismutase (SOD) production was stimulated, but it was principally extracellular in S. aureus. Escherichia coli, a species resistant to the antibiotic, did not reduce Q1 and showed lower superoxide anion generation; besides, there was an increase of intracellular SOD with extracellular decrease.
P M Bogdanov; M M Bertorello; I Albesa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  244     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-23     Completed Date:  1998-04-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  561-6     Citation Subset:  IM    
Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
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MeSH Terms
Anti-Bacterial Agents / metabolism*,  pharmacology*
Drug Resistance, Microbial
Escherichia coli / metabolism
Isoxazoles / metabolism*,  pharmacology*
Naphthols / metabolism*,  pharmacology*
Oxidative Stress / drug effects
Oxygen Consumption / drug effects
Spectrometry, Fluorescence
Staphylococcus aureus / drug effects*,  metabolism*
Superoxide Dismutase / biosynthesis
Superoxides / metabolism
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Isoxazoles; 0/Naphthols; 11062-77-4/Superoxides; 81620-91-9/2-hydroxy-N-(3,4-dimethyl-5-isoxazolyl)-1,4-naphthoquinone imine; EC Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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