Document Detail


Oxidative stress during post-hypoxic-ischemic reperfusion in the newborn lamb: the effect of nitric oxide synthesis inhibition.
MedLine Citation:
PMID:  9078529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Post-hypoxic-ischemic (HI) reperfusion induces endothelium and neurons to produce excessive amounts of nitric oxide and superoxide, leading to peroxynitrite formation, release of protein-bound metal ions (i.e. iron), and cytotoxic oxidants. We produced severe HI in 18 newborn lambs and serially determined plasma prooxidants (non-protein-bound iron), lipid peroxidation (malondialdehyde), and antioxidative capacity [ratio of ascorbic acid/dehydroascorbic acid (AA/DHA), alpha-tocopherol, sulfhydryl groups, allantoin/uric acid ratio, and vitamin A] in blood effluent from the brain before and at 15, 60, 120, and 180 min after HI. The lambs were divided in three groups: six received a placebo (CONT), six received low dose (10 mg/kg/i.v.) N omega-nitro-L-arginine (NLA-10) to block nitric oxide production, and six received high dose NLA (40 mg/kg/i.v.; NLA-40), immediately after completion of HI. Non-protein-bound iron increased in all groups after HI but was significantly lower in both NLA groups at 180 min post-HI (p < 0.05), the AA/DHA ratio showed a consistent decrease in CONT (at 60 min post-HI, p < 0.05), but remained stable in NLA lambs. alpha-Tocopherol decreased steadily in the CONT, but not in the NLA lambs [180 post-H: 1.9 +/- 0.9 versus 4.2 +/- 0.7 microM (NLA-40), p < 0.05). Malondialdehyde was significantly higher in CONT lambs 120 min post-H compared with NLA groups [0.61 +/- 017 versus 0.44 +/- 0.05 microM (NLA-40), p < 0.05]. Vitamin A and sulfhydryl groups did not differ among groups. We conclude that post-H inhibition of nitric oxide synthesis diminishes non-protein-bound iron increment and preserves antioxidant capacity.
Authors:
C A Dorrepaal; F van Bel; R M Moison; M Shadid; M van de Bor; P Steendijk; H M Berger
Related Documents :
18504969 - Redox reactions of phenazine antibiotics with ferric (hydr)oxides and molecular oxygen.
11478249 - Evidence for simultaneous abiotic-biotic oxidations in a microbial-fenton's system.
9705749 - Reduction of oxidation during the preparation of dna and analysis of 8-hydroxy-2'-deoxy...
15067689 - Selective site-specific fenton oxidation of methionine in model peptides: evidence for ...
14630099 - Application of biological processes for the removal of arsenic from groundwaters.
2333289 - Mechanism of the selective functionalization of saturated hydrocarbons by gif systems: ...
19545619 - Nitric oxide bioactivity of traditional chinese medicines used for cardiovascular indic...
18364359 - Phenolic lipids synthesized by type iii polyketide synthase confer penicillin resistanc...
22416889 - Regiodivergent synthesis of functionalized indene derivatives via pt-catalyzed rautenst...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  41     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-07-01     Completed Date:  1997-07-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  321-6     Citation Subset:  IM    
Affiliation:
Department of Pediatrics (Division of Neonatology), Leiden University Hospital, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antioxidants / metabolism
Brain Ischemia / drug therapy*
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Enzyme Inhibitors / therapeutic use*
Hypoxia, Brain / drug therapy*
Lipid Peroxidation / drug effects
Nitric Oxide Synthase / antagonists & inhibitors*
Nitroarginine / therapeutic use*
Oxidation-Reduction
Oxidative Stress / drug effects
Reperfusion Injury / drug therapy*
Sheep
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Enzyme Inhibitors; 2149-70-4/Nitroarginine; EC 1.14.13.39/Nitric Oxide Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  5-Hydroxytryptamine-induced vasoconstriction after cerebral hematoma in piglets.
Next Document:  The angiogenesis inhibitor TNP-470 reduces the growth rate of human neuroblastoma in nude rats.