| Oxidative stress defense in human-skin-derived mesenchymal stem cells versus human keratinocytes: Different mechanisms of protection and cell selection. | |
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MedLine Citation:
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PMID: 20541604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stem cells are undifferentiated cells with the capacity for self-renewal and differentiation. Here we have determined the susceptibility to oxidative stress of isolated mesenchymal stem cells from human skin (S-MSCs) in comparison with keratinocytes, which are differentiated cells of the same lineage. To induce pro-oxidant conditions, S-MSCs and keratinocytes were exposed to 0.5mM H(2)O(2) for 2 h, with oxidative effects analyzed after 4, 12, 24, and 48 h of recovery, in terms of cell growth, vitality, apoptosis, DNA damage, variations in individual antioxidant defense and total oxyradical scavenging capacity toward peroxyl and hydroxyl radicals. The data indicate different abilities across these two cell types to counteract this oxidative stress, which reflects stress that would normally be experienced by these cells under basal conditions. Human keratinocytes seem to have much greater antioxidant defense to counteract the oxidative injury to which they are continuously exposed in the skin. The S-MSCs are surrounded by a complex microenvironment that protects them from external insults, and so they do not have a particularly efficient defense system, and they were generally less responsive to enhanced pro-oxidant challenge. S-MSCs seem particularly prone to apoptotic events, which might thus represent their primary defense mechanism against stress. |
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Authors:
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M Orciani; S Gorbi; M Benedetti; G Di Benedetto; M Mattioli-Belmonte; F Regoli; R Di Primio |
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Publication Detail:
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Type: Journal Article Date: 2010-06-10 |
Journal Detail:
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Title: Free radical biology & medicine Volume: 49 ISSN: 1873-4596 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 830-8 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Histology Section, Department of Molecular Pathology and Innovative Therapies, Marche Polytechnic University, 60020 Ancona, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology Cell Separation Cell Survival / drug effects Cells, Cultured Cytoprotection / physiology Humans Hydrogen Peroxide / pharmacology Immunity, Cellular / drug effects, physiology* Keratinocytes / drug effects, immunology, metabolism, physiology* Mesenchymal Stem Cells / cytology, drug effects, immunology, physiology* Oxidative Stress / drug effects, immunology* Skin* / cytology, immunology |
| Chemical | |
Reg. No./Substance:
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7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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