Document Detail


Oxidative stress contributes to silica nanoparticle-induced cytotoxicity in human embryonic kidney cells.
MedLine Citation:
PMID:  19401228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to elucidate the nanoparticle-induced cytotoxicity and its mechanism, the effects of 20 and 50 nm silica nanoparticles on cultured human embryonic kidney (HEK293) cells were investigated. Cell viability, mitochondrial function, cell morphology, reactive oxygen species (ROS), glutathione (GSH), thiobarbituric acid reactive substance (TBARS), cell cycle and apoptosis were assessed under control and silica exposed conditions. Exposure to 20 or 50 nm SiO(2) nanoparticles at dosage levels between 20 and 100 microg/ml decreased cell viability in a dose-dependent manner. Median lethal dose (LD(50)) of 24h exposure was 80.2+/-6.4 and 140.3+/-8.6 microg/ml for 20 and 50 nm SiO(2) nanoparticles, respectively. Morphological examination revealed cell shrinkage and nuclear condensation after SiO(2) nanoparticle exposure. Increase in intracellular ROS level and reduction in GSH content were also observed in SiO(2) nanoparticle-exposed HEK293 cells. Increase in the amount of TBARS suggested an elevated level of lipid peroxidation. Flow cytometric analysis showed that SiO(2) nanoparticles can cause G2/M phase arrest and apoptotic sub-G1 population increase in a dose-dependent manner. In summary, exposure to SiO(2) nanoparticles resulted in a dose-dependent cytotoxicity in cultured HEK293 cells that was associated with increased oxidative stress.
Authors:
Fen Wang; Feng Gao; Minbo Lan; Huihui Yuan; Yongping Huang; Jianwen Liu
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Publication Detail:
Type:  Journal Article     Date:  2009-05-03
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  23     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-06     Completed Date:  2009-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  808-15     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutics, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / drug effects
Cell Line
Cell Survival / drug effects*
Dose-Response Relationship, Drug
Glutathione / drug effects,  metabolism
Humans
Kidney / cytology,  drug effects,  metabolism
Lethal Dose 50
Lipid Peroxidation / drug effects
Nanoparticles*
Oxidative Stress / drug effects*
Particle Size
Reactive Oxygen Species / metabolism
Silicon Dioxide / administration & dosage,  toxicity*
Time Factors
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; 70-18-8/Glutathione; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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