Document Detail


Oxidative stress and autologous immunoglobulin G binding to band 3 dimers in newborn erythrocytes.
MedLine Citation:
PMID:  16520242     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since birth-induced oxidative stress (OS) results in the removal of erythrocytes from the blood stream, we studied the binding of autologous IgG to erythrocyte band 3 dimers (the 170-kDa band, which marks the erythrocytes for removal) in preterm and term newborns and in adults. The 170-kDa band was present in as much as 74% of preterm, in 21% of term newborns, and in 10% of adults. During erythrocyte ageing "in vitro" (0, 24, and 48 h aerobic incubation), the appearance of the band occurred much faster with erythrocytes from newborns (particularly preterm) than with those from adults. When the blots for the 170-kDa band were quantified by scanning densitometry, it was seen that the 0 time values were significantly higher in preterm compared to term and adult values. After aerobic incubation a progressive increase in the optical density was observed in each group and the densities were higher in preterm than in the other groups. The course of iron release during the various incubations was analogous to that of the 170-kDa band blots, and significant correlations were found at 0 and 48 h. Methemoglobin formation roughly paralleled iron release. Esterified F(2)-isoprostanes (markers of OS) and O(2)(-) production in the nonincubated (0 time) erythrocytes were much higher in newborn (preterm and term) than in adult erythrocytes. Plasma free F(2)-isoprostanes were significantly higher in preterms than in terms and in terms than in adults. Plasma non-protein-bound iron (NPBI) was higher in preterm than in term newborns and not detectable in adults. In conclusion dimers of band 3 with autologous IgG are found under conditions in which OS can be detected in erythrocytes or in plasma: namely in newborns or in aged erythrocytes.
Authors:
Viviana Rossi; Silvia Leoncini; Cinzia Signorini; Giuseppe Buonocore; Patrizia Paffetti; Donatella Tanganelli; Lucia Ciccoli; Mario Comporti
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-12-27
Journal Detail:
Title:  Free radical biology & medicine     Volume:  40     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-07     Completed Date:  2006-07-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  907-15     Citation Subset:  IM    
Affiliation:
Department of Pathophysiology, Experimental Medicine and Public Health, University of Siena, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Anion Exchange Protein 1, Erythrocyte / immunology*
Autoantibodies / analysis*
Deferoxamine / metabolism
Dimerization
Erythrocytes / immunology*,  metabolism
F2-Isoprostanes / blood,  metabolism
Female
Humans
Immunoglobulin G / analysis*
Infant, Newborn
Iron / blood,  metabolism
Male
Methemoglobin / metabolism
Oxidative Stress*
Chemical
Reg. No./Substance:
0/Anion Exchange Protein 1, Erythrocyte; 0/Autoantibodies; 0/F2-Isoprostanes; 0/Immunoglobulin G; 70-51-9/Deferoxamine; 7439-89-6/Iron; 9008-37-1/Methemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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