Document Detail

Oxidative interactions of synthetic lung epithelial lining fluid with metal-containing particulate matter.
MedLine Citation:
PMID:  11557584     Owner:  NLM     Status:  MEDLINE    
Epidemiology studies show association of morbidity and mortality with exposure to ambient air particulate matter (PM). Metals present in PM may catalyze oxidation of important lipids and proteins present in the lining of the respiratory tract. The present study investigated the PM-induced oxidation of human bronchoalveolar lavage (BAL) fluid (BALF) and synthetic lung epithelial lining fluid (sELF) through the measurement of oxygen incorporation and antioxidant depletion assays. Residual oil fly ash (ROFA), an emission source PM that contains approximately 10% by weight of soluble transition metals, was added (0-200 microg/ml) to BALF or sELF and exposed to 20% (18)O(2) (24 degrees C, 4 h). Oxygen incorporation was quantified as excess (18)O in the dried samples after incubation. BALF and diluted sELF yielded similar results. Oxygen incorporation was increased by ROFA addition and was enhanced by ascorbic acid (AA) and mixtures of AA and glutathione (GSH). AA depletion, but not depletion of GSH or uric acid, occurred in parallel with oxygen incorporation. AA became inhibitory to oxygen incorporation when it was present in high enough concentrations that it was not depleted by ROFA. Physiological and higher concentrations of catalase, superoxide dismutase, and glutathione peroxidase had no effect on oxygen incorporation. Both protein and lipid were found to be targets for oxygen incorporation; however, lipid appeared to be necessary for protein oxygen incorporation to occur. Based on these findings, we predict that ROFA would initiate significant oxidation of lung lining fluids after in vivo exposure and that AA, GSH, and lipid concentrations of these fluids are important determinants of this oxidation.
G Sun; K Crissman; J Norwood; J Richards; R Slade; G E Hatch
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  281     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-09-14     Completed Date:  2001-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L807-15     Citation Subset:  IM    
Curriculum in Toxicology, The University of North Carolina at Chapel Hill, 27599, USA.
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MeSH Terms
Antioxidants / pharmacology
Ascorbic Acid / pharmacology
Bronchoalveolar Lavage Fluid / cytology*
Carbon / metabolism,  pharmacology
Dose-Response Relationship, Drug
Glutathione / pharmacology
Metals / metabolism*,  pharmacology*
Oxygen Isotopes / pharmacokinetics
Particulate Matter
Respiratory Mucosa / cytology,  metabolism*
Reg. No./Substance:
0/Antioxidants; 0/Metals; 0/Oxygen Isotopes; 0/Particulate Matter; 0/oil fly ash; 50-81-7/Ascorbic Acid; 70-18-8/Glutathione; 7440-44-0/Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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