Document Detail

Oxidative glycation and free radical production: a causal mechanism of diabetic complications.
MedLine Citation:
PMID:  1649079     Owner:  NLM     Status:  MEDLINE    
Glucose may oxidise under physiological conditions and lead to the production of protein reactive ketoaldehydes, hydrogen peroxide and highly reactive oxidants. Glucose is thus able to modify proteins by the attachment of its oxidation derived aldehydes, leading to the development of novel protein fluorophores, as well as fragment protein via free radical mechanisms. The fragmentation of protein by glucose is inhibitable by metal chelators such as diethylenetriamine pentaacetic acid (DETAPAC) and free radical scavengers such as benzoic acid, and sorbitol. The enzymic antioxidant, catalase, also inhibits protein fragmentation. Protein glycation and protein oxidation are inextricably linked. Indeed, using boronate affinity chromatography to separate glycated from non-glycated material, we demonstrate that proteins which are glycated exhibit an enhanced tryptophan oxidation. Our observation that both glycation and oxidation occur simultaneously further supports the hypothesis that tissue damage associated with diabetes and ageing has an oxidative origin.
J V Hunt; S P Wolff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research communications     Volume:  12-13 Pt 1     ISSN:  8755-0199     ISO Abbreviation:  Free Radic. Res. Commun.     Publication Date:  1991  
Date Detail:
Created Date:  1991-08-22     Completed Date:  1991-08-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709453     Medline TA:  Free Radic Res Commun     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  115-23     Citation Subset:  IM    
Department of Clinical Pharmacology, University College London.
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MeSH Terms
Aging / metabolism
Aldehydes / metabolism
Benzoates / pharmacology
Benzoic Acid
Chelating Agents / pharmacology
Chromatography, Affinity
Diabetes Complications*
Diabetes Mellitus / metabolism
Free Radical Scavengers
Free Radicals*
Glucose / metabolism*
Hydrogen Peroxide / metabolism
Hydroxides / metabolism
Hydroxyl Radical
Pentetic Acid / pharmacology
Proteins / metabolism
Sorbitol / pharmacology
Reg. No./Substance:
0/Aldehydes; 0/Benzoates; 0/Chelating Agents; 0/Free Radical Scavengers; 0/Free Radicals; 0/Hydroxides; 0/Proteins; 3352-57-6/Hydroxyl Radical; 50-70-4/Sorbitol; 50-99-7/Glucose; 65-85-0/Benzoic Acid; 67-43-6/Pentetic Acid; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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