| Oxidative effects of nanosecond pulsed electric field exposure in cells and cell-free media. | |
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MedLine Citation:
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PMID: 22910297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nanosecond pulsed electric field (nsPEF) is a novel modality for permeabilization of membranous structures and intracellular delivery of xenobiotics. We hypothesized that oxidative effects of nsPEF could be a separate primary mechanism responsible for bioeffects. ROS production in cultured cells and media exposed to 300-ns PEF (1-13 kV/cm) was assessed by oxidation of 2',7'-dichlorodihydrofluoresein (H(2)DCF), dihidroethidium (DHE), or Amplex Red. When a suspension of H(2)DCF-loaded cells was subjected to nsPEF, the yield of fluorescent 2',7'-dichlorofluorescein (DCF) increased proportionally to the pulse number and cell density. DCF emission increased with time after exposure in nsPEF-sensitive Jurkat cells, but remained stable in nsPEF-resistant U937 cells. In cell-free media, nsPEF facilitated the conversion of H(2)DCF into DCF. This effect was not related to heating and was reduced by catalase, but not by mannitol or superoxide dismutase. Formation of H(2)O(2) in nsPEF-treated media was confirmed by increased oxidation of Amplex Red. ROS increase within individual cells exposed to nsPEF was visualized by oxidation of DHE. We conclude that nsPEF can generate both extracellular (electrochemical) and intracellular ROS, including H(2)O(2) and possibly other species. Therefore, bioeffects of nsPEF are not limited to electropermeabilization; concurrent ROS formation may lead to cell stimulation and/or oxidative cell damage. |
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Authors:
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Olga N Pakhomova; Vera A Khorokhorina; Angela M Bowman; Raminta Rodaitė-Riševičienė; Gintautas Saulis; Shu Xiao; Andrei G Pakhomov |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-08-15 |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 527 ISSN: 1096-0384 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-09-26 Completed Date: 2012-12-03 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 55-64 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Inc. All rights reserved. |
Affiliation:
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Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells Cell Line Cell Membrane / metabolism Cell Membrane Permeability* Cell Survival Cell-Free System / metabolism Cricetinae Electroporation* / methods Fluoresceins / analysis, metabolism Fluorescent Dyes / analysis, metabolism Humans Hydrogen Peroxide / metabolism Jurkat Cells Oxidation-Reduction Oxidative Stress* Reactive Oxygen Species / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA125482/CA/NCI NIH HHS; R01 GM088303/GM/NIGMS NIH HHS; R01CA125482/CA/NCI NIH HHS; R01GM088303/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Fluorescent Dyes; 0/Reactive Oxygen Species; 76-54-0/2',7'-dichlorofluorescein; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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