| Oxidative damage to DNA in diabetes mellitus. | |
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MedLine Citation:
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PMID: 8618487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Increased production of reactive oxygen species (ROS) and lipid peroxidation may contribute to vascular complications in diabetes. to test whether DNA is also oxidatively damaged in diabetes, we measured 8-hydroxydeoxyguanosine (8-OHdG), an indicator of oxidative damage of DNA, in mononuclear cells. METHODS: For this laboratory-based study, 12 patients with insulin-dependent diabetes mellitus (IDDM) and 15 patients with non-insulin-dependent diabetes mellitus (NIDDM) were matched by age with ten healthy volunteers each. DNA was extracted from mononuclear cells from whole blood. 8-OHdG was assayed by high-pressure liquid chromatography, and ROS were assayed by chemiluminescence. FINDINGS: IDDM and NIDDM patients had significantly higher median concentrations (p , 0.001, U test) of 8-OHdG in their mononuclear cells than their corresponding controls (in fmol/micrograms DNA): 128.2 (interquartile range 96.0-223.2) and 95.2 (64.0-133.5) vs 28.2 (21.7-43.4) and 21.9 (18.0-24.4), respectively. ROS generation by mononuclear cells was also significantly greater (p < 0.01) in diabetic patients than in their controls (in mV): 238.0 (107.0-243.0) and 101.3 (66.0-134.0) vs 69.5 (49.8-91.9) and 56.0 (38.8-62.5), respectively. INTERPRETATION: IDDM and NIDDM patients showed greater oxidative damage to DNA, with increased generation of ROS, than controls. Such changes might contribute to accelerated aging and atherogenesis in diabetes and to the microangiopathic complications of the disease. |
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Authors:
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P Dandona; K Thusu; S Cook; B Snyder; J Makowski; D Armstrong; T Nicotera |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Lancet Volume: 347 ISSN: 0140-6736 ISO Abbreviation: Lancet Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-06-13 Completed Date: 1996-06-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985213R Medline TA: Lancet Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 444-5 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Millard Fillmore Hospitals, Buffalo, NY 14209, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Case-Control Studies Chemiluminescent Measurements Chromatography, High Pressure Liquid DNA / chemistry DNA Damage* Deoxyguanosine / analogs & derivatives*, analysis Diabetes Mellitus, Type 1 / metabolism* Diabetes Mellitus, Type 2 / metabolism* Diabetic Angiopathies / etiology Humans Leukocytes, Mononuclear / chemistry, metabolism Lipid Peroxidation Middle Aged Reactive Oxygen Species / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/8-hydroxy-2'-deoxyguanosine; 0/Reactive Oxygen Species; 9007-49-2/DNA; 961-07-9/Deoxyguanosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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