Document Detail


Oxidative burst and cognate redox signalling reported by luciferase imaging: identification of a signal network that functions independently of ethylene, SA and Me-JA but is dependent on MAPKK activity.
MedLine Citation:
PMID:  11123796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recognition of avirulent microbial pathogens activates an oxidative burst leading to the accumulation of reactive oxygen intermediates (ROIs), which are thought to integrate a diverse set of defence mechanisms resulting in the establishment of plant disease resistance. A novel transgenic Arabidopsis line containing a gst1:luc transgene was developed and employed to report the temporal and spatial dynamics of ROI accumulation and cognate redox signalling in response to attempted infection by avirulent strains of Pseudomonas syringae pv. tomato (Pst). Strong engagement of the oxidative burst was dependent on the presence of functional Pst hrpS and hrpA gene products. Experiments employing pharmacological agents suggested that at least two distinct sources, including an NADPH oxidase and a peroxidase-type enzyme, contributed to the generation of redox cues. The analysis of gst1 and pal1 gene expression in nahG, coi1 and etr1 plants suggested that engagement of the oxidative burst and cognate redox signalling functioned independently of salicylic acid, methyl jasmonate and ethylene. In contrast, studies using a panel of protein kinase and phosphatase inhibitors and in-gel kinase assays in these mutant backgrounds suggested that a 48 kDa mitogen-activated protein kinase (MAPK) activity was required for the activation of gst1 and pal1 in response to redox cues. Thus the engagement of a bifurcating redox signalling pathway possessing a MAPK module may contribute both to the establishment of plant disease resistance, and to the development of cellular protectant mechanisms.
Authors:
J J Grant; B W Yun; G J Loake
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  24     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-02     Completed Date:  2001-03-08     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  569-82     Citation Subset:  IM    
Affiliation:
Institute of Cell and Molecular Biology, University of Edinburgh, Daniel Rutherford Building, King's Buildings, Edinburgh EH9 3JH, UK.
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MeSH Terms
Descriptor/Qualifier:
Acetates / pharmacology
Arabidopsis / genetics,  metabolism,  microbiology
Cyclopentanes / pharmacology
Enzyme Inhibitors / pharmacology
Ethylenes / pharmacology
Flavonoids / pharmacology
Gene Expression Regulation, Plant
Genetic Markers
Glutathione Transferase / genetics,  metabolism
Hydrogen Peroxide / metabolism
Luciferases / genetics,  metabolism
Microscopy, Fluorescence
Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors,  metabolism
Oxidation-Reduction
Oxygen / metabolism*
Oxylipins
Plants, Genetically Modified
Pseudomonas / growth & development
Reactive Oxygen Species / metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Salicylic Acid / pharmacology
Signal Transduction* / drug effects
Time Factors
Chemical
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Acetates; 0/Cyclopentanes; 0/Enzyme Inhibitors; 0/Ethylenes; 0/Flavonoids; 0/Genetic Markers; 0/Oxylipins; 0/Reactive Oxygen Species; 0/Recombinant Fusion Proteins; 1211-29-6/methyl jasmonate; 69-72-7/Salicylic Acid; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; 91GW059KN7/ethylene; EC 1.13.12.-/Luciferases; EC 2.5.1.18/Glutathione Transferase; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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