Document Detail


Oxidative Stress Reduced The Mechanotransduction sensitivity of Porcine Angular Aqueous Plexus Cells To Elevation Of Pressure.
MedLine Citation:
PMID:  24576879     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Purpose:To investigate effect of pressure/flow on the global protein expression profiles of normal and senescent porcine aqueous humour plexus (AAP) cells, which are the porcine equivalent of human Schlemm's canal. Methods:AAP cells were grown for two weeks in physiological (5% O2) or hyperoxic conditions (40% O2) to model cell senescence. Control and senescent AAP cells were subjected to control and elevated hydrostatic pressure gradient of 10 mmHg. Hydraulic conductivity (HC) and transendothelial electric resistance (TEER) was measured. The expression of senescence-associated beta-galactosidase and DNA damage marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) was monitored, and the protein expression profile was analyzed by iTRAQ proteomics method. The expression levels of cytoskeleton and barrier proteins were confirmed by western blot. Results:After 14 days of hyperoxia AAP cells stained positive for 8-OHdG and β-galactosidase. Pressure elevation/flow resulted in significant increase of HC in control cells (1.64 ± 0.18 uL/mmHg/min/cm2, p<0.05), but not in senescent cells (1.34 ± 0.12 uL/mmHg/min/cm2). TEER changes were consistent with the HC results. Proteomics analysis showed that the number of differentially expressed proteins significantly reduced in senescent cells (482 vs. 277 proteins, p<0.05). Cluster of Orthologous Groups analysis further revealed that pressure elevation/flow affected less protein functional groups in senescent cells. The proteins identified by proteomics was verified by western blot analysis, including myosin light chain, claudin-5, ve-cadherin which were significantly reduced in control cells but not in senescent cells. Conclusions:AAP cells are mechano-sensitive, however, cell senescence rendered the cells less responsive to mechanical stimulus which may have pathological consequences.
Authors:
Yuan Lei; W Daniel Stamer; Jihong Wu; Xinghuai Sun
Related Documents :
25015789 - Inhibition of glucose turnover by 3-bromopyruvate counteracts pancreatic cancer stem ce...
23799549 - The effect of mapk inhibitors and ros modulators on cell growth and death of h2o2‑treat...
24083669 - Effect of ursolic acid on mapk in cyclin d1 signaling and ring-type e3 ligase (scf e3s)...
24198279 - Cell delamination in the mesencephalic neural fold and its implication for the origin o...
24687489 - Discovery of novel cell wall-active compounds using p ywac, a sensitive reporter of cel...
24799859 - Rabbit antiserum to mouse embryonic stem cells delays compaction of mouse preimplantati...
19267359 - Improved cell sensitivity and longevity in a rapid impedance-based toxicity sensor.
23800749 - Ros-induced autophagy in cancer cells assists in evasion from determinants of immunogen...
14597189 - Exploring the mechanism of competence development in escherichia coli using quantum dot...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-2-27
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  -     ISSN:  1552-5783     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparison of two methods to measure macular pigment optical density in healthy subjects.
Next Document:  Characterization of Vitamin D Production by Human Ocular Barrier Cells.