Document Detail

Oxidative DNA damage in male Wistar rats exposed to di-n-butyl phthalate.
MedLine Citation:
PMID:  12079616     Owner:  NLM     Status:  MEDLINE    
Dialkyl phthalate esters are used in the plastic industry and widely distributed in the environment. Previously, it has been shown that di-n-butyl phthalate (DBP) produces testicular atrophy and liver enlargement in rodents, and the mechanisms behind this could involve reactive oxygen species (ROS). In this study, oxidative DNA damage was measured in terms of the premutagenic modified nucleoside 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxodG) in nuclear DNA from liver, kidneys, and testes from rats exposed to DBP in the perinatal or preadult period. In one experiment, pregnant rats were administered 0 or 0.5 g DBP/kg/d by gavage from d 7 after conception to d 17 after delivery and organs from male offspring were analyzed. In a second experiment, 25-d-old rats were administered 0, 0.5, or 2 g DBP/kg/d by gavage for 10 d. After perinatal exposure, body and organ weights were unchanged. The 8-oxodG/10(6) dG ratio in liver DNA increased significantly in the exposed group. In contrast, the 8-oxodG/10(6) dG ratio was significantly decreased in kidney DNA, whereas it remained unchanged in the testis. After preadult exposure (postnatal d 25 to 34) the testes weight of the exposed animals were significantly decreased and severe atrophy of the seminiferous tubules was observed. The body weight of the animals in the high-dose group was significantly decreased compared to the control. The 8-oxodG levels in liver, kidney, and testis DNA remained unchanged. Although ROS has been suspected of being involved in the formation of testicular atrophy in phthalate-exposed rats, no apparent sign of oxidative DNA damage was found after phthalate exposure perinatally or during the preadult stage. With respect to phthalate-induced oxidative DNA damage in the liver, it appears that the developmental stage during exposure is important.
A Wellejus; M Dalgaard; S Loft
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of toxicology and environmental health. Part A     Volume:  65     ISSN:  1528-7394     ISO Abbreviation:  J. Toxicol. Environ. Health Part A     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-24     Completed Date:  2002-07-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100960995     Medline TA:  J Toxicol Environ Health A     Country:  England    
Other Details:
Languages:  eng     Pagination:  813-24     Citation Subset:  IM    
Institute of Public Health, University of Copenhagen, Copenhagen, Denmark.
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MeSH Terms
Carcinogens, Environmental / administration & dosage,  toxicity*
DNA Damage / drug effects*
Deoxyguanosine / analogs & derivatives,  metabolism*
Dibutyl Phthalate / administration & dosage,  toxicity*
Environmental Exposure / adverse effects*
Kidney / drug effects,  metabolism,  pathology
Liver / drug effects*,  metabolism,  pathology
Maternal Exposure / adverse effects*
Rats, Wistar
Reactive Oxygen Species / metabolism
Testis / drug effects*,  metabolism,  pathology
Reg. No./Substance:
0/Carcinogens, Environmental; 0/Reactive Oxygen Species; 84-74-2/Dibutyl Phthalate; 961-07-9/Deoxyguanosine

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