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Oxidative Bioconversion of Cholesterol by Pseudomonas sp. Strain ST-200 in a Water-Organic Solvent Two-Phase System.
MedLine Citation:
PMID:  16349329     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Pseudomonas sp. strain ST-200, which is capable of conversion of cholesterol, was isolated from humus soil. This organism effectively modified cholesterol dissolved in an organic solvent by dehydrogenation and oxygenation. When the organism was grown in a medium overlaid with a 10% volume of a mixed organic solvent (p-xylene and diphenylmethane; 3:7, vol/vol) containing cholesterol (20 mg/ml), the cholesterol concentration in the organic solvent was reduced to only 0.4 mg/ml after 8 days. Although the organism did not assimilate cholesterol, 98% of the cholesterol initially present disappeared. The organic solvent layer contained two major and three minor compounds converted from cholesterol. The major compounds were 6beta-hydroxycholest-4-en-3-one (8.9 mg/ml) and cholest-4-ene-3,6-dione (7.6 mg/ml). The concentrations of these compounds were equivalent to 43 and 37% of the cholesterol initially present. This organism would provide an effective and convenient system to oxidize the C-3 and -6 positions of cholesterol by introduction of a hydroxyl or ketone group.
R Aono; N Doukyu; H Kobayashi; H Nakajima; K Horikoshi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  60     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-06-25     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2518-23     Citation Subset:  -    
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Yokohama 227, Japan.
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