Document Detail


Oxidation of cis-5-unsaturated fatty acids in intact rat liver mitochondria: the operation of reduction pathways.
MedLine Citation:
PMID:  7755586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The metabolism of cis-5 unsaturated fatty acids was studied in intact rat liver mitochondria to assess the operation of a reduction pathway. By using direct quantification of metabolites with a capillary-column gas chromatography, 3-hydroxydodecanoate was identified among other metabolites when cis-5-dodecenoate was metabolized in intact rat liver mitochondria. The formation of 3-hydroxydodecanoate supports the existence of a reduction pathway in the metabolism of cis-5-unsaturated fatty acids. This metabolite cannot be produced from the conventional isomerase-mediated pathway. However, the data also indicated the possible operation of the conventional isomerase-mediated pathway in intact rat liver mitochondria. The reduction pathway appears to account for at least 61% of the pathway for cis-5-dodecenoate. This reduction pathway was likely to proceed from the dehydrogenation to trans-2,cis-5-dodecadienoyl-CoA, which was isomerized to delta 3, delta 5-dodecadienoyl-CoA, then to trans-2,trans-4-dodecadienoate. The reduction was mediated by 2,4-dienoyl-CoA reductase by the conversion of trans-2,trans-4-dodecadienoyl-CoA into trans-3-dodecenoyl-CoA. However, direct reduction of the cis-5 double bond was also shown to be operating, although to a lesser extent.
Authors:
K Y Tserng; S J Jin
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  308 ( Pt 1)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-06-22     Completed Date:  1995-06-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  39-44     Citation Subset:  IM    
Affiliation:
Medical Research Service, VA Medical Center, Cleveland, OH, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fatty Acids, Monounsaturated / metabolism
Fatty Acids, Unsaturated / metabolism*
Mitochondria, Liver / metabolism*
NADP / metabolism
Oxidation-Reduction
Oxygen Consumption
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
DK-45040/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Monounsaturated; 0/Fatty Acids, Unsaturated; 2430-94-6/5-dodecenoic acid; 53-59-8/NADP
Comments/Corrections

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