Document Detail


Oxidation of cell wall polysaccharides by hydrogen peroxide: a potential mechanism for cell wall breakdown in plants.
MedLine Citation:
PMID:  3800997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Incubation of cellulose, sodium carboxylcellulose, pectin, polygalacturonic acid, xylan and arabinogalactan with hydrogen peroxide (0.1-10 mM) resulted in rapid breakdown of the polysaccharides when measured by a reduction of solution viscosity or an increase in reducing groups. When the reaction mixtures were precipitated with ethanol or fractionated on G-25-300 Sephadex, low molecular weight reducing groups increased with incubation time indicating that polymer cleavage was occurring and not simply polymer modification. Oxidation was most rapid at pH 6.5 or 7.5, although secondary optima between pH 3.5 and 5.5 were also observed, depending on the polysaccharide. Purified cell walls isolated from various organs of tomato, cucumber and soybean were similarly degraded and the ethanol-soluble reaction products were partially characterized. The data support the hypothesis that hydrogen peroxide generated by peroxidase from NADH may play a role during cell wall breakdown in plants.
Authors:
A R Miller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  141     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-02-04     Completed Date:  1987-02-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  238-44     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Catalysis
Cell Wall*
Chemical Phenomena
Chemistry
Hydrogen Peroxide*
Hydrogen-Ion Concentration
Molecular Weight
Plant Physiological Phenomena*
Polysaccharides*
Chemical
Reg. No./Substance:
0/Polysaccharides; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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