Document Detail

Oxidation and carboxy methyl lysine-modification of albumin: possible involvement in the progression of oxidative stress in hemodialysis patients.
MedLine Citation:
PMID:  16671336     Owner:  NLM     Status:  MEDLINE    
Hemodialysis (HD) patients are frequently in a state of increased oxidative stress, and hyperglycemia appears to be a major factor. We recently found that oxidized human serum albumin (HSA) is a reliable marker of oxidative stress in HD patients. However, the issue of whether oxidized HSA is associated with the progression of oxidative stress in HD patients with or without diabetes is not clear. In the present study, we examined the effect of a qualitative modification of HSA in HD patients with or without diabetes. Blood samples from 10 HD patients with diabetes, 7 HD patients without diabetes, and 10 healthy age-matched controls were examined. The increase in plasma protein carbonyl content and advanced glycation endproducts (AGEs) in HD patients was largely due to an increase in the levels of oxidized HSA. Furthermore, these increases were greatest in HD patients with diabetes. Purified HSA from HD patients (non-DM-HSA) was carbonylated and AGE-modified. The amount of modified HSA was the highest in HD patients with diabetes (DM-HSA). Carboxy methyl lysine (CML)-modified HSA triggered a neutrophil respiratory burst, and this activity was closely correlated with the increase in the CML/HSA ratio. These findings indicate that uremia plays an important role in the progression of oxidative stress in HD patients via an increase in CML-modified HSA. They also indicate that diabetic complications further exacerbate the progression of oxidative stress by further increasing the amount of these modified HSA molecules.
Katsumi Mera; Makoto Anraku; Kenichiro Kitamura; Keisuke Nakajou; Toru Maruyama; Kimio Tomita; Masaki Otagiri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hypertension research : official journal of the Japanese Society of Hypertension     Volume:  28     ISSN:  0916-9636     ISO Abbreviation:  Hypertens. Res.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2006-05-04     Completed Date:  2006-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9307690     Medline TA:  Hypertens Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  973-80     Citation Subset:  IM    
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
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MeSH Terms
Aged, 80 and over
Arginine / analogs & derivatives,  metabolism
Blood Proteins / metabolism
Case-Control Studies
Diabetes Mellitus / metabolism,  therapy
Glycosylation End Products, Advanced / metabolism
Imidazoles / metabolism
Kidney Failure, Chronic / metabolism*
Lysine / analogs & derivatives,  metabolism
Middle Aged
Neutrophils / metabolism
Norleucine / analogs & derivatives,  metabolism
Oxidative Stress / physiology*
Protein Carbonylation
Pyrroles / metabolism
Renal Dialysis*
Respiratory Burst
Serum Albumin / metabolism*
Reg. No./Substance:
0/Blood Proteins; 0/Glycosylation End Products, Advanced; 0/Imidazoles; 0/Pyrroles; 0/Serum Albumin; 0/imidazolone; 124505-87-9/pentosidine; 5157-09-5/Norleucine; 56-87-1/Lysine; 5746-04-3/N(6)-carboxymethyllysine; 74-79-3/Arginine; 74509-14-1/2-formyl-5-(hydroxymethyl)pyrrole-1-norleucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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