| Oxidation of bovine serum albumin initiated by the Fenton reaction--effect of EDTA, tert-butylhydroperoxide and tetrahydrofuran. | |
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MedLine Citation:
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PMID: 16517506 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidation of bovine serum albumin (BSA) was investigated using different oxidants: The water-soluble azo-initiator 2,2'azo-bis-(2-amidinopropane) hydrochloride (AAPH), a combination of FeCl(3) and ascorbate or the Fenton oxidant consisting of FeCl(2), H(2)O(2) and EDTA. In addition, the effects of exogenous compounds such as tert-butyl hydroperoxide (tBuOOH) or solvents such as tetrahydrofuran (THF), often used in model systems, was evaluated. The extent of protein damage was studied by measuring protein carbonyl groups and protein hydroperoxides. The interaction between Fenton oxidant and EDTA, THF or tBuOOH was further characterized using spin trapping electron spin resonance (ESR) spectroscopy. The results showed that the extent of protein oxidation depended on the oxidant used. The Fenton oxidant was the most reactive of the initiators tested. However, in the absence of EDTA, the Fenton system produced protein carbonyl groups on BSA equivalent to that obtained with the other oxidants, however, significantly more protein hydroperoxide was produced. Surprisingly, it was also found that addition of tBuOOH or THF to BSA reduced protein damage when the oxidation was initiated with the Fenton oxidant. ESR investigation showed that EDTA played a key role in the generation of free radicals. It was also revealed that in an EDTA containing system both tBuOOH and THF were able to react with radicals without inducing protein damage in effect protecting BSA from oxidative damage. |
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Authors:
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Caroline P Baron; Hanne H F Refsgaard; Leif H Skibsted; Mogens L Andersen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical research Volume: 40 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 409-17 Citation Subset: IM |
Affiliation:
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Department of Seafood Research, Danish Institute for Fisheries Research, Technical University of Denmark, Building 221, Kgs, Lyngby DK-2800, Denmark. cba@dfu.min.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Edetic Acid / pharmacology* Electron Spin Resonance Spectroscopy Free Radicals Furans / pharmacology* Hydrogen Peroxide Iron Oxidation-Reduction / drug effects Oxidative Stress / physiology* Serum Albumin, Bovine / chemistry*, drug effects Spin Labels tert-Butylhydroperoxide / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Fenton's reagent; 0/Free Radicals; 0/Furans; 0/Serum Albumin, Bovine; 0/Spin Labels; 109-99-9/tetrahydrofuran; 60-00-4/Edetic Acid; 7439-89-6/Iron; 75-91-2/tert-Butylhydroperoxide; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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