| Oxidation of acetate in rabbit skeletal muscle: detection by 13C NMR spectroscopy in vivo. | |
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MedLine Citation:
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PMID: 8875417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The results of a proton-decoupled and Overhauser-enhanced 13C NMR study of acetate metabolism in skeletal muscle are reported. [2-13C]Acetate was infused intravenously over 2 h into anesthetized rabbits, and skeletal muscle in the lateral thigh was monitored by 13C NMR spectroscopy at 4.7 T. Stable 13C enrichment in carbons 2, 3, and 4 of glutamate was observed at the end of the infusion, and the half-time for enrichment was 17 min for glutamate C4 and 50 min for glutamate C2 and C3. The contribution of exogenous acetate to acetylcoenzyme A was nearly equal in skeletal muscle and heart in vivo (83-87%, measured in tissue extracts), comparable with earlier perfused heart studies in which acetate was the sole available substrate. Although relative flux through the combined anaplerotic pathways (relative to citric acid cycle flux) was higher in quiescent skeletal muscle (28%) compared with hearts (3%) from the same animals, actual anaplerotic flux was estimated to be substantially higher in heart than in skeletal muscle after correcting for differences in citric acid cycle flux in the two tissues. |
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Authors:
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L Szczepaniak; E E Babcock; C R Malloy; A D Sherry |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 36 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 1996 Sep |
Date Detail:
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Created Date: 1997-04-17 Completed Date: 1997-04-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 451-7 Citation Subset: IM |
Affiliation:
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Department of Radiology, Mary Nell and Ralph B. Rogers Magnetic Resonance Center, University of Texas Southwestern Medical Center, Dallas, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism* Carbon Isotopes Magnetic Resonance Spectroscopy Muscles / metabolism* Oxidation-Reduction* |
| Grant Support | |
ID/Acronym/Agency:
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HL34557/HL/NHLBI NIH HHS; P41-RR02584/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Carbon Isotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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