Document Detail

Oxidant-mediated epithelial cell injury in idiopathic pulmonary fibrosis.
MedLine Citation:
PMID:  3034979     Owner:  NLM     Status:  MEDLINE    
Lung inflammatory cells of patients with idiopathic pulmonary fibrosis (IPF) were evaluated for their ability to injure 51Cr-labeled AKD alveolar epithelial cells in the presence and absence of IPF alveolar epithelial lining fluid (ELF). The IPF cells were spontaneously releasing exaggerated amounts of superoxide (O.2) and hydrogen peroxide (H2O2) compared with normal (P less than 0.02). Cytotoxicity of the AKD cells was markedly increased when the IPF inflammatory cells were incubated with autologous ELF (P less than 0.02). The majority of IPF patients had ELF myeloperoxidase levels above normal (P less than 0.002). Incubation of IPF ELF with AKD cells in the presence of H2O2 caused increased cellular injury (P less than 0.01 compared with control), which was suppressed by methionine, a myeloperoxidase system scavenger. IPF patients with high concentrations of ELF myeloperoxidase deteriorated more rapidly than those with low ELF myeloperoxidase (P less than 0.05). Thus, IPF is characterized by an increased spontaneous production of oxidants by lung inflammatory cells, the presence of high concentrations of myeloperoxidase in the ELF of the lower respiratory tract, and a synergistic cytotoxic effect of alveolar inflammatory cells and ELF on lung epithelial cells, suggesting oxidants may play a role in causing the epithelial cell injury of this disorder.
A M Cantin; S L North; G A Fells; R C Hubbard; R G Crystal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  79     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-07-10     Completed Date:  1987-07-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1665-73     Citation Subset:  AIM; IM    
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MeSH Terms
Body Fluids / pathology*
Epithelium / pathology
Hydrogen Peroxide / secretion*
Macrophages / pathology,  secretion*
Middle Aged
Neutrophils / pathology,  secretion*
Peroxidase / secretion*
Pulmonary Alveoli / pathology*
Pulmonary Fibrosis / pathology*
Superoxides / secretion*
Reg. No./Substance:
11062-77-4/Superoxides; 7722-84-1/Hydrogen Peroxide; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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