| Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor. | |
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MedLine Citation:
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PMID: 18205808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells treated with low doses of linoleic acid hydroperoxide (LoaOOH) exhibit a cell-cycle delay that may provide a mechanism to overcome oxidative stress. Strains sensitive to LoaOOH from the genome-wide deletion collection were screened to identify deletants in which the cell-cycle delay phenotype was reduced. Forty-seven deletants were identified that were unable to mount the normal delay response, implicating the product of the deleted gene in the oxidant-mediated cell-cycle delay of the wild-type. Of these genes, SWI6 was of particular interest due to its role in cell-cycle progression through Start. The swi6 deletant strain was delayed on entry into the cell cycle in the absence of an oxidant, and oxidant addition caused no further delay. Transforming the swi6 deletant with SWI6 on a plasmid restored the G1 arrest in response to LoaOOH, indicating that Swi6p is involved in oxidant sensing leading to cell division delay. Micro-array studies identified genes whose expression in response to LoaOOH depended on SWI6. The screening identified 77 genes that were upregulated in the wild-type strain and concurrently downregulated in the swi6 deletant treated with LoaOOH. These data show that functions such as heat shock response, and glucose transport are involved in the response. |
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Authors:
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Chii Shyang Fong; Mark D Temple; Nazif Alic; Joyce Chiu; Moritz Durchdewald; Geoffrey W Thorpe; Vincent J Higgins; Ian W Dawes |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-14 |
Journal Detail:
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Title: FEMS yeast research Volume: 8 ISSN: 1567-1356 ISO Abbreviation: FEMS Yeast Res. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-23 Completed Date: 2008-11-17 Revised Date: 2009-07-24 |
Medline Journal Info:
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Nlm Unique ID: 101085384 Medline TA: FEMS Yeast Res Country: England |
Other Details:
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Languages: eng Pagination: 386-99 Citation Subset: IM |
Affiliation:
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Ramaciotti Centre for Gene Function Analysis and School of Biotechnology and Biomolecular Sciences, UNSW, NSW, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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drug effects* Glycolysis Linoleic Acids / pharmacology* Lipid Peroxides / pharmacology* Oxidative Stress Saccharomyces cerevisiae / cytology*, genetics, metabolism Saccharomyces cerevisiae Proteins / physiology* Signal Transduction Transcription Factors / physiology* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Linoleic Acids; 0/Lipid Peroxides; 0/SWI6 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 0/YAP1 protein, S cerevisiae; 25657-09-4/linoleic acid hydroperoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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