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Oxamflatin improves developmental competence of porcine somatic cell nuclear transfer embryos.
MedLine Citation:
PMID:  23013534     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract Aberrant epigenetic nuclear reprogramming of somatic nuclei is a major cause of low success in cloning. It has been demonstrated that treatment of histone deacetylase inhibitors (HDACi) enhances developmental potential of somatic cell nuclear transfer (SCNT) embryos by alteration of epigenetic status. The aim of the present study was to investigate the effect of oxamflatin, a novel HDACi, on the developmental competence of porcine SCNT embryos. Treatment with 1 μM oxamflatin for 9 h after activation of SCNT embryos increased both in vitro and in vivo developmental competence. Treatment of SCNT embryos with 1 μM oxamflatin significantly increased blastocyst rate and total cell number in blastocysts (33.3±6.0 and 73.1±1.6, respectively) than that of controls (10.3±3.7 and 54.1±3.5, respectively) or scriptaid (16.4±4.6 and 64.4±2.1, respectively). Moreover, oxamflatin showed significant higher overall cloning efficiency from 0.9% to 3.2%, whereas scriptaid demonstrated 0% to 1.8%. In conclusion, these results indicate that oxamflatin treatment improves the developmental competence of porcine SCNT embryos.
Authors:
Sol-Ji Park; Hee-Jung Park; Ok-Jae Koo; Woo-Jae Choi; Joon-Ho Moon; Dae-Kee Kwon; Jung-Taek Kang; Sujin Kim; Ji-Yei Choi; Goo Jang; Byeong-Chun Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular reprogramming     Volume:  14     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  398-406     Citation Subset:  IM    
Affiliation:
1 Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University , Seoul 151-742, Korea.
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