| Oxalate uptake in fetal rat myoblasts. | |
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MedLine Citation:
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PMID: 9604814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxalate uptake was studied in fetal rat myoblasts to attempt to define the characteristics of oxalate transport in myocardial cells. Oxalate uptake was found to be time, temperature and pH dependent and was inhibited by metabolic inhibitors (Dinitrophenol and iodoacetamide). Inhibition of oxalate uptake by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) suggested transport by an anion exchange mechanism. Both chloride and sulfate inhibited oxalate uptake suggesting competition by both ions for a combined transporter. Although medium calcium concentration and calcium ionophores had no effect on oxalate uptake, the calcium channel blocker, nifedipine, significantly decreased oxalate uptake. Diacylglycerol (DAG) stimulated oxalate uptake, while forskolin had no effect. These studies suggest that myocardial cells transport oxalate by a mechanism similar to that described in renal epithelial cells. The uptake of oxalate by myocardial cells raises questions about the physiologic role of oxalate in myocardial cells and the mechanism of cardiac problems in primary hyperoxaluria. |
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Authors:
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A C Eusebio; H E Williams |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of nephrology Volume: 11 Suppl 1 ISSN: 1121-8428 ISO Abbreviation: J. Nephrol. Publication Date: 1998 Mar-Apr |
Date Detail:
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Created Date: 1998-08-06 Completed Date: 1998-08-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9012268 Medline TA: J Nephrol Country: ITALY |
Other Details:
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Languages: eng Pagination: 60-2 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University of California, Davis School of Medicine, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Cells, Cultured Fetal Heart / cytology, metabolism Hydrogen-Ion Concentration Hyperoxaluria, Primary / metabolism Myocardium / cytology, metabolism* Oxalates / pharmacokinetics* Rats Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Oxalates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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