Document Detail

Oxalate transport in a line of porcine renal epithelial cells--LLC-PK1 cells.
MedLine Citation:
PMID:  8201674     Owner:  NLM     Status:  MEDLINE    
The present studies examined oxalate handling in LLC-PK1 cells, an epithelial cell line of porcine origin. These cells appear to express transport systems for oxalate, as evidenced by the fact that uptake was saturable, time dependent and sensitive to the anion transport inhibitor DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid). Oxalate uptake in these cells was also affected by the presence of certain inorganic anions (Cl-, SO4(2-), or HCO3-) but not by organic anions (para-aminohippurate, urate, malate, phenylsuccinate, succinate). This uptake was Na independent and unaffected by changes in membrane potential but was affected by external pH, with acidic pH stimulating and alkaline pH inhibiting oxalate accumulation. These findings suggest that LLC-PK1 cells express oxalate transporters similar to those observed in the mammalian renal cortex. Further studies using these cells may prove useful in defining the conditions that govern transcellular oxalate flux in renal epithelial cells.
S Ebisuno; H Koul; M Menon; C Scheid
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of urology     Volume:  152     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-07-01     Completed Date:  1994-07-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-42     Citation Subset:  AIM; IM    
Division of Urology, Minami Wakayama National Hospital, Japan.
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MeSH Terms
Biological Transport / physiology
Cell Line
Epithelial Cells
Hydrogen-Ion Concentration
Kidney / cytology,  metabolism*
Oxalates / metabolism*
Grant Support
Reg. No./Substance:

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