Document Detail


Oxalate transport in a line of porcine renal epithelial cells--LLC-PK1 cells.
MedLine Citation:
PMID:  8201674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present studies examined oxalate handling in LLC-PK1 cells, an epithelial cell line of porcine origin. These cells appear to express transport systems for oxalate, as evidenced by the fact that uptake was saturable, time dependent and sensitive to the anion transport inhibitor DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid). Oxalate uptake in these cells was also affected by the presence of certain inorganic anions (Cl-, SO4(2-), or HCO3-) but not by organic anions (para-aminohippurate, urate, malate, phenylsuccinate, succinate). This uptake was Na independent and unaffected by changes in membrane potential but was affected by external pH, with acidic pH stimulating and alkaline pH inhibiting oxalate accumulation. These findings suggest that LLC-PK1 cells express oxalate transporters similar to those observed in the mammalian renal cortex. Further studies using these cells may prove useful in defining the conditions that govern transcellular oxalate flux in renal epithelial cells.
Authors:
S Ebisuno; H Koul; M Menon; C Scheid
Related Documents :
9713974 - Biological effects and cellular uptake of c-myc antisense oligonucleotides and their ca...
11948234 - A role for co(2) and bicarbonate transporters in metabolic exchanges in the brain.
20687544 - Synthesis and in vitro evaluation of defined hpma folate conjugates: influence of aggre...
16971494 - Unique uptake and efflux systems of inorganic phosphate in osteoclast-like cells.
7254224 - Induction of sister-chromatid exchanges and cell cycle delay in cultured mammalian cell...
7814974 - Radiation-induced transformation of sv40-immortalized human thyroid epithelial cells by...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of urology     Volume:  152     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-07-01     Completed Date:  1994-07-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-42     Citation Subset:  AIM; IM    
Affiliation:
Division of Urology, Minami Wakayama National Hospital, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / physiology
Cell Line
Epithelial Cells
Hydrogen-Ion Concentration
Kidney / cytology,  metabolism*
Oxalates / metabolism*
Swine
Grant Support
ID/Acronym/Agency:
DK43184/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Oxalates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Colonization of urinary catheters by Escherichia coli and Providencia stuartii in a laboratory model...
Next Document:  Tumor associated glycoprotein-72 is highly expressed in prostatic adenocarcinomas.