Document Detail

Oxalate metabolism by the acetogenic bacterium Moorella thermoacetica.
MedLine Citation:
PMID:  14769464     Owner:  NLM     Status:  MEDLINE    
Whole-cell and cell-extract experiments were performed to study the mechanism of oxalate metabolism in the acetogenic bacterium Moorella thermoacetica. In short-term, whole-cell assays, oxalate consumption was low unless cell suspensions were supplemented with CO(2), KNO(3), or Na(2)S(2)O(3). Cell extracts catalyzed the oxalate-dependent reduction of benzyl viologen. Oxalate consumption occurred concomitant to benzyl viologen reduction; when benzyl viologen was omitted, oxalate was not appreciably consumed. Based on benzyl viologen reduction, specific activities of extracts averaged 0.6 micromol oxalate oxidized min(-1) mg protein(-1). Extracts also catalyzed the formate-dependent reduction of NADP(+); however, oxalate-dependent reduction of NADP(+) was negligible. Oxalate- or formate-dependent reduction of NAD(+) was not observed. Addition of coenzyme A (CoA), acetyl-CoA, or succinyl-CoA to the assay had a minimal effect on the oxalate-dependent reduction of benzyl viologen. These results suggest that oxalate metabolism by M. thermoacetica requires a utilizable electron acceptor and that CoA-level intermediates are not involved.
Steven L Daniel; Christine Pilsl; Harold L Drake
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  231     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-10     Completed Date:  2004-06-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  39-43     Citation Subset:  IM    
Department of Biological Sciences, Eastern Illinois University, Charleston, IL 61920, USA.
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MeSH Terms
Acetates / metabolism
Electron Transport
Energy Metabolism*
Gram-Positive Bacteria / growth & development,  metabolism*
Oxalates / metabolism*
Reg. No./Substance:
0/Acetates; 0/Oxalates

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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