Document Detail


Ovum pick-up in sheep: efficiency of in vitro embryo production, vitrification and birth of offspring.
MedLine Citation:
PMID:  10735116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The production of offspring involving available technologies like ovum pick-up, in vitro embryo production and cryopreservation has not been fully described in the sheep. We tested the overall efficiency of these procedures on 20 Sarda dairy ewes that were twice stimulated for recovery of follicular oocytes. In total, 415 oocytes were aspirated from 522 follicles (11.5 oocytes/ewe), and 328 of them (9.1 oocytes/ewe) were selected for in vitro embryo production procedure. Development into blastocysts occurred in 98 embryos (2.7 blastocysts/ewe), of which 64 were vitrified and 34 were transferred, in pairs, directly to recipients. The pregnancy rate, diagnosed at 80 d for fresh and vitrified embryos, did not differ significantly (47.1 vs 42.8%, respectively), but there were significant differences in lambing rates between the 2 groups (41.2 vs 23.8%, respectively). Overall, 24 lambs were born; all weighed within the range for the breed, but head deformities were observed in 2 cases. The results of this study show that with application of the above techniques, it is possible to obtain repeatedly embryos and viable offspring.
Authors:
G Ptak; M Dattena; P Loi; M Tischner; P Cappai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Theriogenology     Volume:  52     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  1999 Oct 
Date Detail:
Created Date:  2000-04-19     Completed Date:  2000-04-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1105-14     Citation Subset:  IM    
Affiliation:
Sardinian Institute of Zootechnics, Sassari, Italy. gptak@tiscalinet.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology
Embryo Transfer / veterinary*
Female
Fertilization in Vitro / veterinary*
Oocyte Donation / veterinary*
Oocytes / cytology*
Ovarian Follicle / cytology
Pregnancy
Pregnancy Outcome / veterinary
Sheep
Zygote / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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