| Ovum pick-up in sheep: efficiency of in vitro embryo production, vitrification and birth of offspring. | |
| | |
MedLine Citation:
|
PMID: 10735116 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The production of offspring involving available technologies like ovum pick-up, in vitro embryo production and cryopreservation has not been fully described in the sheep. We tested the overall efficiency of these procedures on 20 Sarda dairy ewes that were twice stimulated for recovery of follicular oocytes. In total, 415 oocytes were aspirated from 522 follicles (11.5 oocytes/ewe), and 328 of them (9.1 oocytes/ewe) were selected for in vitro embryo production procedure. Development into blastocysts occurred in 98 embryos (2.7 blastocysts/ewe), of which 64 were vitrified and 34 were transferred, in pairs, directly to recipients. The pregnancy rate, diagnosed at 80 d for fresh and vitrified embryos, did not differ significantly (47.1 vs 42.8%, respectively), but there were significant differences in lambing rates between the 2 groups (41.2 vs 23.8%, respectively). Overall, 24 lambs were born; all weighed within the range for the breed, but head deformities were observed in 2 cases. The results of this study show that with application of the above techniques, it is possible to obtain repeatedly embryos and viable offspring. |
| | |
Authors:
|
G Ptak; M Dattena; P Loi; M Tischner; P Cappai |
Related Documents
:
|
115656 - The nature and role of pregnancy-associated antigens and the endocrinology of early pre... 6643756 - Effects of maternal nutrition on the initiation of secondary wool follicles in foetal s... 16725906 - A definition of the breeding season of pool dorset ewes. 7991796 - Effect of post-ovulation nutritional status in ewes on early conceptus survival and gro... 8555086 - The prenatal identification of fetal compatibility in neonatal alloimmune thrombocytope... 15733266 - A randomized trial comparing alternative approaches to prenatal diagnosis counseling in... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Theriogenology Volume: 52 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 1999 Oct |
Date Detail:
|
Created Date: 2000-04-19 Completed Date: 2000-04-19 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1105-14 Citation Subset: IM |
Affiliation:
|
Sardinian Institute of Zootechnics, Sassari, Italy. gptak@tiscalinet.it |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blastocyst / cytology Embryo Transfer / veterinary* Female Fertilization in Vitro / veterinary* Oocyte Donation / veterinary* Oocytes / cytology* Ovarian Follicle / cytology Pregnancy Pregnancy Outcome / veterinary Sheep Zygote / cytology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pregnancy-associated glycoprotein levels in pregnant goats inoculated with Toxoplasma gondii or List...
Next Document: Effect of time during transport of excised mare ovaries on oocyte recovery rate and quality after in...