Document Detail

Ovulation detection and timing of insemination based on hormone concentrations, vaginal cytology and the endoscopic appearance of the vagina in domestic bitches.
MedLine Citation:
PMID:  2621729     Owner:  NLM     Status:  MEDLINE    
The fertilization period, the time when fertilization can occur, can best be determined using a combination of vaginal cytology and morphology of the vaginal mucosa, behaviour and endocrinology. Neither vaginal cytological changes nor standing oestrus are reliable indicators of the fertilization period per se although with natural mating or when using AI with fresh semen there may be enough biological leeway for pregnancy to follow insemination timed using these parameters alone. This is indicated by the well recognized fecundity of bitches mated early or late in oestrus. Precise discrimination of the fertilization period, which is often required and is necessary for AI using frozen semen, can be achieved by using vaginoscopy to detect the onset of shrinkage with angulation of the mucosal folds. Vaginoscopy is also useful to determine the probable end of the fertilization period and the transition to a 'metoestrous' vaginal smear is also useful in this regard. Detection of the LH peak enables the start of the fertilization period to be forecast quite precisely but, in lieu of practical assays with which to measure plasma LH, a rise in plasma progesterone concentration to 6-8 ng/ml may be more practical.
I A Jeffcoate; F E Lindsay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility. Supplement     Volume:  39     ISSN:  0449-3087     ISO Abbreviation:  J. Reprod. Fertil. Suppl.     Publication Date:  1989  
Date Detail:
Created Date:  1990-03-28     Completed Date:  1990-03-28     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  0225652     Medline TA:  J Reprod Fertil Suppl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  277-87     Citation Subset:  IM    
Department of Veterinary Physiology, University of Glasgow Veterinary School, UK.
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MeSH Terms
Dogs / physiology*
Estradiol / blood
Insemination / physiology*
Luteinizing Hormone / blood
Ovulation Detection / methods*
Progesterone / blood
Time Factors
Vagina / cytology
Grant Support
//Wellcome Trust
Reg. No./Substance:
50-28-2/Estradiol; 57-83-0/Progesterone; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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