Document Detail

Ovine vascular endothelial growth factor: nucleotide sequence and expression in fetal tissues.
MedLine Citation:
PMID:  9777367     Owner:  NLM     Status:  MEDLINE    
To examine the role of vascular endothelial growth factor (VEGF) in mediating angiogenesis and vascular permeability during fetal development, we determined the gene expression of VEGF in ovine fetal tissues. Further, we cloned and sequenced the ovine VEGF cDNA encoding VEGF164 from sheep placenta. VEGF protein was localized in epithelial cells of the placenta and fetal kidney, and in hepatocytes of the fetal liver. By Northern analysis, a major VEGF mRNA species of 3.7 kb was identified in all tissues examined, with abundance highest in the lung and lowest in the liver. The most prominent molecular form expressed in ovine fetal tissues appeared to be VEGF164 with low levels of expression of VEGF120 and VEGF188. Cloning and sequence analysis of the most abundant form of ovine VEGF cDNA in the placenta confirmed the prediction of a 164-amino acid peptide, with a putative N-terminal signal sequence of 26 amino acids. Comparison of the VEGF cDNA sequence among different species revealed that VEGF is highly conserved suggesting an important role in development.
C Y Cheung; R A Brace
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Growth factors (Chur, Switzerland)     Volume:  16     ISSN:  0897-7194     ISO Abbreviation:  Growth Factors     Publication Date:  1998  
Date Detail:
Created Date:  1998-12-18     Completed Date:  1998-12-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9000468     Medline TA:  Growth Factors     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  11-22     Citation Subset:  IM    
Department of Reproductive Medicine, University of California, San Diego School of Medicine, La Jolla 92093-0802, USA.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
Cloning, Molecular
DNA, Complementary
Endothelial Growth Factors / analysis,  chemistry,  genetics*
Fetus / metabolism*
Gene Expression Regulation, Developmental*
Liver / embryology,  metabolism
Lung / embryology,  metabolism
Lymphokines / analysis,  chemistry,  genetics*
Molecular Sequence Data
Placenta / metabolism
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sheep / embryology*,  metabolism
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Grant Support
Reg. No./Substance:
0/DNA, Complementary; 0/Endothelial Growth Factors; 0/Lymphokines; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors

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