Document Detail


Ovine corticotropin-releasing factor and vasopressin: antibody-quenching studies on hypothalamic extracts of normal and Brattleboro rats.
MedLine Citation:
PMID:  6605243     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stalk median eminence (SME) extracts were preincubated with antibodies to ovine corticotropin-releasing factor (oCRF) and/or vasopressin, and the resulting CRF bioactivity tested with the isolated anterior pituitary cell column bioassay. The ACTH-releasing ability of Wistar rat SME was reduced by 60% with vasopressin antiserum, by 53% with oCRF antiserum, and by 81% after incubation with both antisera simultaneously. SME-stimulated LH release was unaffected by these antisera, which were all used at a dilution of 1:1000. The ACTH-releasing activity of SME could not be completely abolished by increasing the oCRF antibody concentration, or, in the case of ovine SME, by decreasing the tissue concentration preincubated with oCRF antibodies. With Brattleboro SME (which contains no endogenous vasopressin) ACTH-releasing activity was reduced by 37%, 51%, and 57% with anti-oCRF at dilutions of 1:5000, 1:1000, and 1:500, respectively, but could not be reduced further by more concentrated antisera. We conclude, therefore, that the CRF bioactivity of rat SME is probably not due solely to an oCRF-like peptide, but that other substances, one being vasopressin, contribute to its ACTH-releasing ability.
Authors:
E A Linton; G E Gillies; P J Lowry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  113     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1983-12-17     Completed Date:  1983-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1878-83     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Corticotropin-Releasing Hormone / immunology,  physiology*
Female
Hypothalamus / analysis*
Pituitary Gland, Anterior / analysis
Rats
Rats, Brattleboro
Rats, Inbred Strains
Sheep
Vasopressins / immunology,  physiology*
Chemical
Reg. No./Substance:
0/Antibodies; 11000-17-2/Vasopressins; 9015-71-8/Corticotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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