Document Detail


Ovine surgical model of uterine space restriction: interactive effects of uterine anomalies and multifetal gestations on fetal and placental growth.
MedLine Citation:
PMID:  20574052     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intrauterine growth restriction (IUGR) is observed in conditions with limitations in uterine space (e.g., uterine anomalies and multifetal gestations). IUGR is associated with reduced fetal weight, organ growth, and a spectrum of adult-onset diseases. To examine the interaction of uterine anomalies and multifetal gestations, we developed a surgical uterine space restriction model with a unilateral uterine horn ligation before breeding (unilateral surgery). Placentas and fetuses were studied on Gestational Day (GD) 120 and GD 130 (term = 147 days). Unilateral surgery decreased placentome numbers in singleton and twin pregnancies (25% and 50%, respectively) but not unilateral triplets. Unilateral surgery decreased total placentome weight in twin pregnancies (decreased 24%). Fetuses categorized as uterine space restricted (unilateral twin and both groups of triplets) had 51% fewer placentomes per fetus and a 31% reduction in placentomal weight per fetus compared to the nonrestricted group (control singleton, unilateral singleton, and control twin). By GD 130, uterine space-restricted fetuses exhibited decreased weight, smaller crown-rump, abdominal girth, and thoracic girth as well as decreased fetal heart, kidney, liver, spleen, and thymus weights. Lung and brain weights were unaffected, demonstrating asymmetric IUGR. At GD 130, placental efficiency (fetal weight per total placentomal weight) was elevated in uterine space-restricted fetuses. However, fetal arterial creatinine, blood urea nitrogen, and cholesterol were elevated, suggesting insufficient placental clearance. Maternal-to-fetal glucose and triglycerides ratios were elevated in the uterine space-restricted pregnancies, suggesting placental nutrient transport insufficiency. This model allows for examination of interactive effects of uterine space restriction-induced IUGR on placental adaptation and fetal organ growth.
Authors:
Katie M Meyer; Jill M Koch; Jayanth Ramadoss; Pamela J Kling; Ronald R Magness
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-23
Journal Detail:
Title:  Biology of reproduction     Volume:  83     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-22     Completed Date:  2011-02-28     Revised Date:  2011-11-01    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  799-806     Citation Subset:  IM    
Affiliation:
Departments of Pediatrics, University of Wisconsin, Madison, Wisconsin 53715, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Crown-Rump Length
Disease Models, Animal*
Female
Fetal Blood
Fetal Development / physiology*
Fetal Growth Retardation / blood,  physiopathology*
Fetal Weight
Gestational Age
Ligation
Organ Size
Placenta / growth & development,  physiopathology
Placentation / physiology*
Pregnancy
Pregnancy, Multiple / physiology*
Sheep, Domestic
Uterus / abnormalities*,  anatomy & histology,  surgery*
Grant Support
ID/Acronym/Agency:
HD38843/HD/NICHD NIH HHS; HL49210/HL/NHLBI NIH HHS; HL87144/HL/NHLBI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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