| Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep. | |
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MedLine Citation:
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PMID: 20382821 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In situ detection of ovine progressive pneumonia virus (OPPV) and the phenotypic identification of the cells that harbor OPPV have not been described for the OPPV-affected tissues, which include lung, mammary gland, synovial membranes of the carpal joint, and choroid plexus of the brain. In this study, the authors first developed a single enzyme-based automated immunohistochemical (IHC) analysis for detection of OPPV capsid antigen (CA) on OPPV-affected tissues, using 2 anti-CAEV CA monoclonal antibodies, 5A1 and 10A1, and 2 enzyme-based IHC systems. Out of 10 naturally and persistently OPPV-infected ewes, OPPV CA was detected in intercellular regions of the carpal synovial membrane of 1 ewe, in cells resembling alveolar macrophages and pulmonary interstitial macrophages in lung tissue of 3 ewes, and in mammary alveolar cells of 1 ewe. Furthermore, dual enzyme-based automated IHC analyses revealed that OPPV CA was predominantly detected in CD172a- or CD163-positive alveolar macrophages of the lungs and mammary gland. That anti-inflammatory (CD163) and downregulatory (CD172a) types of alveolar macrophage harbor OPPV CA leads to the possibility that during persistent infection with OPPV, the host alveolar macrophage might serve to limit inflammation while OPPV persists undetected by the host adaptive immune response in the lung and mammary gland. |
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Authors:
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L M Herrmann-Hoesing; S M Noh; K R Snekvik; S N White; D A Schneider; T Truscott; D P Knowles |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-12-31 |
Journal Detail:
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Title: Veterinary pathology Volume: 47 ISSN: 1544-2217 ISO Abbreviation: Vet. Pathol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-08-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0312020 Medline TA: Vet Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 518-28 Citation Subset: IM |
Affiliation:
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Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-6630, USA. lherrman@vetmed.wsu.edu or lynn.herrmann@ars.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / analysis Antigens, Differentiation, Myelomonocytic / analysis Antigens, Viral / analysis* Capsid / immunology Choroid Plexus / virology Female Lentivirus Infections / immunology, veterinary*, virology Lentiviruses, Ovine-Caprine / immunology* Macrophages, Alveolar / immunology, virology* Mammary Glands, Animal / virology Receptors, Cell Surface / analysis Sheep Sheep Diseases / immunology, virology* Synovial Membrane / virology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/Antigens, Viral; 0/CD163 antigen; 0/Receptors, Cell Surface |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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