| Ovine middle cerebral artery characterization and quantification of ultrastructure and other features: changes with development. | |
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MedLine Citation:
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PMID: 22116510 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Regulation of tone, blood pressure, and blood flow in the cerebral vasculature is of vital importance, particularly in the developing infant. We tested the hypothesis that, in addition to accretion of smooth muscle cells (SMCs) in cell layers with vessel thickening, significant changes in smooth muscle structure, as well as phenotype, extracellular matrix, and membrane proteins, in the media of cerebral arteries (CAs) during the course of late fetal development account for associated changes in contractility. Using transmission electron, confocal, wide-field epifluorescence, and light microscopy, we examined the structure and ultrastructure of CAs. Also, we utilized wire myography, Western immunoblotting, and real-time quantitative PCR to examine several other features of these arteries. We compared the main branch ovine middle CAs of 95- and 140-gestational day (GD) fetuses with those of adults (n = 5 for each experimental group). We observed a graded increase in phenylephrine- and KCl-induced contractile responses with development. Structurally, lumen diameter, media thickness, and media cross-sectional area increased dramatically from one age group to the next. With maturation, the cross-sectional profiles of CA SMCs changed from flattened bands in the 95-GD fetus to irregular ovoid-shaped fascicles in the 140-GD fetus and adult. We also observed a change in the type of collagen, specific integrin molecules, and several other parameters of SMC morphology with maturation. Ovine CAs at 95 GD appeared morphologically immature and poorly equipped to respond to major hemodynamic adjustments with maturation. |
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Authors:
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Ravi Goyal; David A Henderson; Nina Chu; Lawrence D Longo |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-11-23 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 302 ISSN: 1522-1490 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-02-23 Completed Date: 2012-04-17 Revised Date: 2013-04-12 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R433-45 Citation Subset: IM |
Affiliation:
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Center for Perinatal Biology, Loma Linda Univ. School of Medicine, Loma Linda, CA 92350, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Fetus / drug effects, embryology, ultrastructure Male Microscopy, Electron, Transmission Middle Cerebral Artery / anatomy & histology, drug effects, growth & development*, physiology, ultrastructure Muscle, Smooth, Vascular / drug effects, growth & development*, ultrastructure Phenylephrine / pharmacology Potassium Chloride / pharmacology Sheep Sympathomimetics / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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HD-03807/HD/NICHD NIH HHS; R01 HD003807-39/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sympathomimetics; 59-42-7/Phenylephrine; 7447-40-7/Potassium Chloride |
| Comments/Corrections | |
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