Document Detail

Ovine middle cerebral artery characterization and quantification of ultrastructure and other features: changes with development.
MedLine Citation:
PMID:  22116510     Owner:  NLM     Status:  MEDLINE    
Regulation of tone, blood pressure, and blood flow in the cerebral vasculature is of vital importance, particularly in the developing infant. We tested the hypothesis that, in addition to accretion of smooth muscle cells (SMCs) in cell layers with vessel thickening, significant changes in smooth muscle structure, as well as phenotype, extracellular matrix, and membrane proteins, in the media of cerebral arteries (CAs) during the course of late fetal development account for associated changes in contractility. Using transmission electron, confocal, wide-field epifluorescence, and light microscopy, we examined the structure and ultrastructure of CAs. Also, we utilized wire myography, Western immunoblotting, and real-time quantitative PCR to examine several other features of these arteries. We compared the main branch ovine middle CAs of 95- and 140-gestational day (GD) fetuses with those of adults (n = 5 for each experimental group). We observed a graded increase in phenylephrine- and KCl-induced contractile responses with development. Structurally, lumen diameter, media thickness, and media cross-sectional area increased dramatically from one age group to the next. With maturation, the cross-sectional profiles of CA SMCs changed from flattened bands in the 95-GD fetus to irregular ovoid-shaped fascicles in the 140-GD fetus and adult. We also observed a change in the type of collagen, specific integrin molecules, and several other parameters of SMC morphology with maturation. Ovine CAs at 95 GD appeared morphologically immature and poorly equipped to respond to major hemodynamic adjustments with maturation.
Ravi Goyal; David A Henderson; Nina Chu; Lawrence D Longo
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-11-23
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  302     ISSN:  1522-1490     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-23     Completed Date:  2012-04-17     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R433-45     Citation Subset:  IM    
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MeSH Terms
Fetus / drug effects,  embryology,  ultrastructure
Microscopy, Electron, Transmission
Middle Cerebral Artery / anatomy & histology,  drug effects,  growth & development*,  physiology,  ultrastructure
Muscle, Smooth, Vascular / drug effects,  growth & development*,  ultrastructure
Phenylephrine / pharmacology
Potassium Chloride / pharmacology
Sympathomimetics / pharmacology
Grant Support
Reg. No./Substance:
0/Sympathomimetics; 1WS297W6MV/Phenylephrine; 660YQ98I10/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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