| Oviductal cell proteome alterations during the reproductive cycle in pigs. | |
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MedLine Citation:
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PMID: 18540664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mammalian oviduct plays a crucial role in events leading to the establishment of pregnancy. During the reproductive cycle, the reproductive system undergoes various changes, including alterations in the number of different cell types in the oviductal epithelium and changes in the height of oviductal cells. Maintaining the unique oviductal environment required for the fertilization and early embryonic development comes with an energy cost to the organism. Therefore, it is hypothesized that structural and functional changes to the oviduct during the reproductive cycle represent vital preparations for the development of suitable environments for conception and embryo support. Here, we aimed to identify the changes in protein expression profile that occur during the follicular and luteal stages of the reproductive cycle in oviductal epithelial cells. The porcine oviductal epithelial cell proteomes from the follicular and luteal stages of the reproductive cycle were contrasted after separation by 2-D gel electrophoresis. Several oviductal epithelial cell proteins were up- or down-regulated during the reproductive cycle. We checked the quantitative changes of two of these molecules during different stages of the reproductive cycle using Western blot analysis. Finally, a number of these proteins were identified using tandem mass spectrometry. The results demonstrated distinctive differences in the proteomic profiles of the oviduct between follicular and luteal phases of the reproductive cycle. |
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Authors:
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Adil Seytanoglu; A Stephen Georgiou; Edita Sostaric; Paul F Watson; William V Holt; Alireza Fazeli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-10 |
Journal Detail:
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Title: Journal of proteome research Volume: 7 ISSN: 1535-3893 ISO Abbreviation: J. Proteome Res. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-07 Completed Date: 2008-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 2825-33 Citation Subset: IM |
Affiliation:
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Academic Unit of Reproductive and Developmental Medicine, University of Sheffield, Level 4, The Jessop Wing, Tree Root Walk, Sheffield S10 2SF, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Epithelium / metabolism Estrous Cycle Fallopian Tubes / cytology, metabolism* Female Gene Expression Regulation Proteome / metabolism* Reproduction* Swine Tandem Mass Spectrometry |
| Chemical | |
Reg. No./Substance:
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0/Proteome |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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