Document Detail


Overview of cell fractionation.
MedLine Citation:
PMID:  18228352     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell fractionation is a useful preparative and analytical method in cell biology. It is essential for analysis of composition and function of cellular compartments and it is used to prepare materials for in vitro reconstitution studies This overview discusses the basic principles of centrifugation, the instruments available, choice of media, evaluation of fractionation, and procedure optimization.
Authors:
J D Castle
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]     Volume:  Chapter 3     ISSN:  1934-2616     ISO Abbreviation:  Curr Protoc Cell Biol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-01-29     Completed Date:  2008-03-24     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101287856     Medline TA:  Curr Protoc Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 3.1     Citation Subset:  IM    
Affiliation:
University of Virginia, Charlottesville, Virginia, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation / instrumentation,  methods*
Centrifugation / instrumentation,  methods
Centrifugation, Density Gradient / methods
Equipment Design
Eukaryotic Cells / ultrastructure
Organelles

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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