Document Detail

Overproduction of a truncated poly (vinyl alcohol) dehydrogenase in recombinant Pichia pastoris by low-temperature induction strategy and related mechanism analysis.
MedLine Citation:
PMID:  23207825     Owner:  NLM     Status:  Publisher    
Based on the N-terminal sequencing of poly (vinyl alcohol) dehydrogenase (PVADH), a 1,644-bp gene encoding a truncated PVADH (tPVADH) was amplified using the synthetic gene (GenBank accession No. JQ235753) as a template, and was further transformed into Pichia pastoris GS115 with the vector pPIC9K. The maximal tPVADH activity reached 546 U/mL in shake flask. The influence of methanol concentration and induction temperature on tPVADH production was further investigated in 3-L bioreactor. When the methanol concentration and induction temperature were controlled at 15 g/L and 22 °C, respectively, the maximal tPVADH activity reached 8,464 U/mL, which was nearly 10 times that of mature PVADH expressed under the same condition and was the highest level ever reported. The reason responsible for the significant improvement of tPVADH production at low induction temperature was explored in terms of cell viability, extracellular proteases activity, and alcohol oxidase activity.
Dongxu Jia; Long Liu; Huilin Wang; Dongxu Zhang; Jianghua Li; Guocheng Du; Jian Chen
Related Documents :
7756335 - Ethanol-induced aggregation and fusion of small phosphatidylcholine liposome: participa...
10675505 - The lea-like protein hsp 12 in saccharomyces cerevisiae has a plasma membrane location ...
9523305 - Ph-metric logp 10. determination of liposomal membrane-water partition coefficients of ...
6869825 - Physical separation of the aqueous phase and lipoidal lamellae from multilamellar lipos...
8161605 - Mass spectrometry as an aid to the identification of ergots and dihydroergots: comparis...
20126565 - Infrared thermal imaging for detection of peripheral vascular disorders.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Bioprocess and biosystems engineering     Volume:  -     ISSN:  1615-7605     ISO Abbreviation:  Bioprocess Biosyst Eng     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088505     Medline TA:  Bioprocess Biosyst Eng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Differential Pathological Response to Preoperative Chemotherapy across Breast Cancer Intrinsic Subty...
Next Document:  Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueo...