Document Detail


Overproduction of thymidine by recombinant brevibacterium helvolum amplified with thymidine monophosphate phosphohydrolase gene from bacteriophage PBS2.
MedLine Citation:
PMID:  15055759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microbial fermentation process could be used to produce thymidine biologically but many of the enzymes related to nucleotide biosynthesis are highly regulated. To overcome the complex regulation steps, an analogue mutant of Brevibacterium helvolum resistant to fluorouracil, hydroxyurea, and trimethoprim was constructed. This mutant accumulated 380 mg thymidine 1(-1) in 16 h in shake-flask culture. However, the accumulation of thymidine monophosphate (TMP) inside the cells suggested a low activity of nucleotidase which degrades TMP to thymidine. This limitation was overcome by cloning the TMP phosphohydrolase (TMPase) gene of the unusual bacteriophage, PBS2. As a result, TMP in recombinant cells decreased from 230 micromol g(-1) cell to 20 micromol g(-1) cell with accumulation of 500 mg thymidine l(-1) in the medium.
Authors:
Hyeon Cheol Lee; Jung Mi Ahn; Sang Nam Lee; Jung Hoe Kim
Related Documents :
16209559 - Heuristic optimization of antibody production by chinese hamster ovary cells.
16086249 - Characteristics of fed-batch cultures of recombinant escherichia coli containing human-...
10833229 - Reduced growth yield of activated sludge in organic protonophore-containing batch culture.
20309539 - High-level production of heterologous proteins using untreated cane molasses and corn s...
7765929 - Applications of improved stoichiometric model in medium design and fed-batch cultivatio...
11375159 - Synthesis and fermentation properties of novel galacto-oligosaccharides by beta-galacto...
8268859 - Evaluation of skin damage of cyclic monoterpenes, percutaneous absorption enhancers, by...
2042649 - Characterization of human renal fibroblasts in health and disease: i. immunophenotyping...
16899119 - Role of chromosome stability and telomere length in the production of viable cell lines...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology letters     Volume:  26     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-04-01     Completed Date:  2004-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  265-8     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry,  genetics*
Bacteriophages / enzymology*,  genetics
Brevibacterium / metabolism*
Chromatography, High Pressure Liquid
Cloning, Molecular
Drug Resistance, Bacterial
Fermentation
Fluorouracil / pharmacology
Hydroxyurea / pharmacology
Mutation
Nucleotidases / genetics*,  metabolism
Phosphoric Monoester Hydrolases / genetics*,  metabolism
Plasmids / metabolism
Recombinant Proteins / metabolism
Thymidine / biosynthesis*,  metabolism
Thymidine Monophosphate / metabolism*
Trimethoprim / pharmacology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Recombinant Proteins; 127-07-1/Hydroxyurea; 365-07-1/Thymidine Monophosphate; 50-89-5/Thymidine; 51-21-8/Fluorouracil; 738-70-5/Trimethoprim; EC 3.1.3.-/Nucleotidases; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.35/thymidylate 5'-phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Disclosure of HIV-positive status to Latino gay men's social networks.
Next Document:  Methyl tert-butyl ether biodegradation by microbial consortia obtained from soil samples of gasoline...