Document Detail


Overlapping functions of different dynamin isoforms in clathrin-dependent and -independent endocytosis in pancreatic beta cells.
MedLine Citation:
PMID:  18442475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previously, we identified a clathrin-dependent slow endocytosis and a clathrin-independent fast endocytosis in pancreatic beta cells, both triggered by elevated cytoplasmic Ca(2+) concentration. In the current study, we attempted to explore the roles of different dynamin isoforms in these endocytotic processes. We first confirmed the existence of both neuron-specific dynamin 1 and ubiquitous dynamin 2 in INS-1 cells using both quantitative RT-PCR and Western blot experiments. By specifically knocking down the endogenous level of either dynamin isoform from INS-1 cells, we showed that dynamin 1 and dynamin 2 simultaneously participate in the clathrin-independent and -dependent membrane retrieval in pancreatic beta cells. Transferrin internalization was also inhibited in cells with knock down of both dynamin 1 and dynamin 2. Based on these results, we argue that different dynamin isoforms play overlapping roles in different types of endocytosis.
Authors:
Jingze Lu; Zixuan He; Junmei Fan; Pingyong Xu; Liangyi Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-28
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  371     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-06-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  315-9     Citation Subset:  IM    
Affiliation:
National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Datun Road 15#, Beijing 100101, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Clathrin / genetics,  metabolism*
Dynamin I / analysis,  genetics,  metabolism*
Dynamin II / analysis,  genetics,  metabolism*
Endocytosis*
Humans
Insulin-Secreting Cells / chemistry,  metabolism,  physiology*
Mice
Protein Isoforms / analysis,  genetics,  metabolism
Chemical
Reg. No./Substance:
0/Clathrin; 0/Protein Isoforms; EC 3.5.1.50/Dynamin I; EC 3.6.5.5/Dynamin II

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