| Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 20944015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Accurate chromosome segregation is dependent on the centromere-specific histone H3 isoform known generally as CenH3, or as Cse4 in budding yeast. Cytological experiments have shown that Cse4 appears at extracentromeric loci in yeast cells deficient for both the CAF-1 and HIR histone H3/H4 deposition complexes, consistent with increased nondisjunction in these double mutant cells. Here, we examined molecular aspects of this Cse4 mislocalization. Genome-scale chromatin immunoprecipitation analyses demonstrated broader distribution of Cse4 outside of centromeres in cac1Δ hir1Δ double mutant cells that lack both CAF-1 and HIR complexes than in either single mutant. However, cytological localization showed that the essential inner kinetochore component Mif2 (CENP-C) was not recruited to extracentromeric Cse4 in cac1Δ hir1Δ double mutant cells. We also observed that rpb1-1 mutants displayed a modestly increased Cse4 half-life at nonpermissive temperatures, suggesting that turnover of Cse4 is partially dependent on Pol II transcription. We used genome-scale assays to demonstrate that the CAF-1 and HIR complexes independently stimulate replication-independent histone H3 turnover rates. We discuss ways in which altered histone exchange kinetics may affect eviction of Cse4 from noncentromeric loci. |
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Authors:
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Jessica Lopes da Rosa; John Holik; Erin M Green; Oliver J Rando; Paul D Kaufman |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-10-13 |
Journal Detail:
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Title: Genetics Volume: 187 ISSN: 1943-2631 ISO Abbreviation: Genetics Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-12 Completed Date: 2011-04-20 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 9-19 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cell Biology, University of California, Berkeley, California 947202, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Centromere / genetics, metabolism* Chromatin Assembly and Disassembly Chromosomal Proteins, Non-Histone / chemistry, genetics, metabolism DNA-Binding Proteins / chemistry, genetics, metabolism Enzyme Activation Genomics Half-Life Histones / metabolism* Humans Kinetochores / metabolism Mutation Nuclear Proteins / metabolism* Protein Stability Protein Transport RNA Polymerase II / metabolism Repressor Proteins / metabolism* Ribonucleases / metabolism* Saccharomyces cerevisiae / cytology*, enzymology, genetics, metabolism* Saccharomyces cerevisiae Proteins / chemistry, genetics, metabolism* Temperature Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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F31 AI 078726/AI/NIAID NIH HHS; R01 GM055712-13/GM/NIGMS NIH HHS; R01 GM079205/GM/NIGMS NIH HHS; R01 GM079205-03/GM/NIGMS NIH HHS; R01 GM079205-04/GM/NIGMS NIH HHS; R01 GM079205-05/GM/NIGMS NIH HHS; R01 GM55712/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CSE4 protein, S cerevisiae; 0/Chromosomal Proteins, Non-Histone; 0/DNA-Binding Proteins; 0/HIR1 protein, S cerevisiae; 0/Histones; 0/Nuclear Proteins; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; EC 2.7.7.-/RNA Polymerase II; EC 3.1.-/Ribonucleases; EC 3.1.13.4/POP2 protein, S cerevisiae |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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