Document Detail


Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  20944015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accurate chromosome segregation is dependent on the centromere-specific histone H3 isoform known generally as CenH3, or as Cse4 in budding yeast. Cytological experiments have shown that Cse4 appears at extracentromeric loci in yeast cells deficient for both the CAF-1 and HIR histone H3/H4 deposition complexes, consistent with increased nondisjunction in these double mutant cells. Here, we examined molecular aspects of this Cse4 mislocalization. Genome-scale chromatin immunoprecipitation analyses demonstrated broader distribution of Cse4 outside of centromeres in cac1Δ hir1Δ double mutant cells that lack both CAF-1 and HIR complexes than in either single mutant. However, cytological localization showed that the essential inner kinetochore component Mif2 (CENP-C) was not recruited to extracentromeric Cse4 in cac1Δ hir1Δ double mutant cells. We also observed that rpb1-1 mutants displayed a modestly increased Cse4 half-life at nonpermissive temperatures, suggesting that turnover of Cse4 is partially dependent on Pol II transcription. We used genome-scale assays to demonstrate that the CAF-1 and HIR complexes independently stimulate replication-independent histone H3 turnover rates. We discuss ways in which altered histone exchange kinetics may affect eviction of Cse4 from noncentromeric loci.
Authors:
Jessica Lopes da Rosa; John Holik; Erin M Green; Oliver J Rando; Paul D Kaufman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-10-13
Journal Detail:
Title:  Genetics     Volume:  187     ISSN:  1943-2631     ISO Abbreviation:  Genetics     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-12     Completed Date:  2011-04-20     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9-19     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cell Biology, University of California, Berkeley, California 947202, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Centromere / genetics,  metabolism*
Chromatin Assembly and Disassembly
Chromosomal Proteins, Non-Histone / chemistry,  genetics,  metabolism
DNA-Binding Proteins / chemistry,  genetics,  metabolism
Enzyme Activation
Genomics
Half-Life
Histones / metabolism*
Humans
Kinetochores / metabolism
Mutation
Nuclear Proteins / metabolism*
Protein Stability
Protein Transport
RNA Polymerase II / metabolism
Repressor Proteins / metabolism*
Ribonucleases / metabolism*
Saccharomyces cerevisiae / cytology*,  enzymology,  genetics,  metabolism*
Saccharomyces cerevisiae Proteins / chemistry,  genetics,  metabolism*
Temperature
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
F31 AI 078726/AI/NIAID NIH HHS; R01 GM055712-13/GM/NIGMS NIH HHS; R01 GM079205/GM/NIGMS NIH HHS; R01 GM079205-03/GM/NIGMS NIH HHS; R01 GM079205-04/GM/NIGMS NIH HHS; R01 GM079205-05/GM/NIGMS NIH HHS; R01 GM55712/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/CSE4 protein, S cerevisiae; 0/Chromosomal Proteins, Non-Histone; 0/DNA-Binding Proteins; 0/HIR1 protein, S cerevisiae; 0/Histones; 0/Nuclear Proteins; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; EC 2.7.7.-/RNA Polymerase II; EC 3.1.-/Ribonucleases; EC 3.1.13.4/POP2 protein, S cerevisiae
Comments/Corrections

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