Document Detail

Overexpression of serpin squamous cell carcinoma antigens in psoriatic skin.
MedLine Citation:
PMID:  11851888     Owner:  NLM     Status:  MEDLINE    
Squamous cell carcinoma antigen belongs to the serpin family and is used for the diagnosis and management of squamous cell carcinoma. We investigated the involvement of squamous cell carcinoma antigen in psoriasis, as it is always detected in the sera of patients with psoriasis. Squamous cell carcinoma antigen localization in psoriatic epidermis varied depending on its concentration in the patient's sera. When its level was low in serum, weak and scattered staining was observed in the granular layer. With a high concentration of squamous cell carcinoma antigen, strong staining through the suprabasal to granular layer and condensed staining around the plasma membrane or intracellular space was detected in the affected epidermis. Interestingly, squamous cell carcinoma antigen was abundant in nuclei of the granular layer cells and elongated rete ridges. Immunoelectron microscopy confirmed the localization of squamous cell carcinoma antigen in the nuclei as well as in the periphery of the cell membrane. A cDNA library was constructed from psoriatic epidermis and both clones, SCCA1 and SCCA2, were obtained. Attempts to raise specific antibodies or to prepare cRNA probes for SCCA1 and SCCA2 were unsuccessful because of their nearly identical structures. A primer pair from each reactive site sequence enabled us to give a distinctive product for SCCA1 and SCCA2 by reverse transcription polymerase chain reaction. Analysis using these primers demonstrated that the SCCA2 transcript was specifically expressed in psoriatic skin tissues. Our results suggest that overexpression of squamous cell carcinoma antigens is associated with the disease activity of psoriasis.
Atsushi Takeda; Dousei Higuchi; Tadahito Takahashi; Masashi Ogo; Peter Baciu; Paul F Goetinck; Toshihiko Hibino
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  118     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-02-19     Completed Date:  2002-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  147-54     Citation Subset:  IM    
Laboratory of Biochemistry, Faculty of Arts and Sciences, Sagami Women's University, Kanagawa, Japan.
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MeSH Terms
Antigens, Neoplasm / genetics,  metabolism*
Cell Nucleus / metabolism
In Situ Hybridization
Microscopy, Immunoelectron
Middle Aged
Psoriasis / metabolism*
RNA, Messenger / metabolism
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
Serpins / metabolism*
Skin / metabolism*
Tissue Distribution
Reg. No./Substance:
0/Antigens, Neoplasm; 0/RNA, Messenger; 0/Serpins; 0/squamous cell carcinoma-related antigen

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