Document Detail


Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome.
MedLine Citation:
PMID:  22772454     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the level of ribosomal RNA (rRNA) in cumulus cells (CCs) from women with polycystic ovary syndrome (PCOS) undergoing ICSI.
MATERIALS AND METHODS: The study population included three healthy oocyte donors (control) and three patients with PCOS. RNA expression in CCs was assessed using quantitative real-time PCR assay to measure the pre-rRNA transcripts (5'ETS region), 18 S, 5.8 S and 28 S rRNA.
RESULTS: The level of 18 S rRNA is 3.9 times higher (p = 0.036) and the level of 5.8 S rRNA is 2.9 times higher (p = 0.049) in CCs of PCOS patients than in CCs of healthy women. The fold change in expression of 28 S rRNA in CCs of PCOS patients also exceeds that in the control group, but did not reach a statistical significance (p = 0.342).
CONCLUSION: Our observations support the idea that CCs of PCOS patients contain comparatively more ribosomes that CCs of healthy oocyte donors that may indicate a higher proliferation rate or up-regulated translation of protein factors in ССs of PCOS patients.
Authors:
Mikhail Polzikov; Sergey Yakovenko; Julia Voznesenskaya; Maria Troshina; Olga Zatsepina
Related Documents :
12111344 - Polymorphism of the vitamin d(3) receptor in patients with psoriasis.
22913794 - Continuous venovenous hemodialysis with regional citrate anticoagulation in patients wi...
20545954 - Homocysteine status and cardiovascular risk factors in patients with psoriasis: a case-...
6476854 - Psoralens and uv-a and uv-b twice weekly for the treatment of psoriasis.
25306224 - Clinical analysis of transvaginal hydrolaparoscopy in infertile patients.
11343644 - Epileptic seizures may begin hours in advance of clinical onset: a report of five patie...
Publication Detail:
Type:  Journal Article     Date:  2012-07-07
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-08     Completed Date:  2013-05-03     Revised Date:  2013-10-11    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1141-5     Citation Subset:  IM    
Affiliation:
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya St, 117997 Moscow, Russia. polzikov@yahoo.co.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Case-Control Studies
Cumulus Cells / physiology*
Female
Gene Expression
Humans
Polycystic Ovary Syndrome / genetics*
RNA, Ribosomal / genetics*
RNA, Ribosomal, 18S
RNA, Ribosomal, 28S
RNA, Ribosomal, 5.8S
Reverse Transcriptase Polymerase Chain Reaction
Sperm Injections, Intracytoplasmic
Chemical
Reg. No./Substance:
0/RNA, Ribosomal; 0/RNA, Ribosomal, 18S; 0/RNA, Ribosomal, 28S; 0/RNA, Ribosomal, 5.8S
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  ESTIMATION OF ABSORBED ORGAN DOSES AND EFFECTIVE DOSE BASED ON BODY MASS INDEX IN DIGITAL RADIOGRAPH...
Next Document:  Increases in [Formula: see text]O(2max) with "live high-train low" altitude training: role of ventil...